Chen Yanfen, Li Mengqiu, Zheng Fanghao, Jiang Wei, Lei Kaijun, Li Huaiguo, Liu Dongwen, Zhang Bairong, He Mingfeng
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China.
Foshan Hospital of Traditional Chinese Medicine, Foshan 528000, Guangdong, China.
Evid Based Complement Alternat Med. 2022 Feb 16;2022:9312611. doi: 10.1155/2022/9312611. eCollection 2022.
Skin and soft tissue infections (SSTIs) are a group of common diseases, usually caused by bacteria. Shangke Huangshui (SK) has been widely used to treat various SSTIs diseases for many years, but its mechanism is unclear. Therefore, this study was designed to investigate the anti-infective effect of SK on different skin and soft tissue infection diseases and to explore its underlying mechanism.
The subcutaneous abscess mouse model, the dermal ulcer rat model, and the infectious soft tissue injury rat model were established by injection of to evaluate the anti-inflammatory and antibacterial effects of SK. Abscess volume, local appearance score and histological changes, bacterial contents, and hydroxyproline concentration in the skin or soft tissue were analyzed. The levels of NO, TNF-, IL-1, and IL-8 in the serum and tissue were determined by ELISA method. The mRNA expression levels of TLR2, MyD88, TAK1, NF-B, AP-1, and other genes were measured with qRT-PCR method, and the protein expression of TLR2, MyD88, TAK1, NF-B, and AP-1 was detected by western blot method.
SK had an obvious therapeutic effect on skin and soft tissue infections. It reduced the volume of abscess and promoted the healing of skin ulcer, improved pathological phenomena, such as inflammatory infiltration of skin and soft tissue, reduced the levels of white blood cells and NO in the blood, decreased bacteria contents in the skin and soft tissue. Furthermore, SK decreased the mRNA expression of TLR2, MyD88, TAK1, NF-B and AP-1, and other related genes and also downregulated the protein expression of TLR2, MyD88, TAK1, NF-B, and AP-1.
The experiments provide evidence that SK can treat skin and soft tissue infection diseases based on its comprehensive antibacterial and anti-inflammatory effects. The therapeutic mechanism may be associated with the inhibition of TLR2/MyD88/NF-B signaling pathway.
皮肤及软组织感染（SSTIs）是一组常见疾病，通常由细菌引起。伤科黄水（SK）多年来已被广泛用于治疗各种皮肤及软组织感染性疾病，但其作用机制尚不清楚。因此，本研究旨在探讨SK对不同皮肤及软组织感染性疾病的抗感染作用，并探索其潜在机制。
通过注射建立皮下脓肿小鼠模型、皮肤溃疡大鼠模型和感染性软组织损伤大鼠模型，以评估SK的抗炎和抗菌作用。分析脓肿体积、局部外观评分和组织学变化、细菌含量以及皮肤或软组织中的羟脯氨酸浓度。采用ELISA法测定血清和组织中NO、TNF-α、IL-1和IL-8的水平。用qRT-PCR法检测TLR2、MyD88、TAK1、NF-κB、AP-1等基因的mRNA表达水平，并用western blot法检测TLR2、MyD88、TAK1、NF-κB和AP-1的蛋白表达。
SK对皮肤及软组织感染有明显的治疗作用。它减小了脓肿体积，促进了皮肤溃疡的愈合，改善了皮肤和软组织炎症浸润等病理现象，降低了血液中的白细胞水平和NO水平，减少了皮肤和软组织中的细菌含量。此外，SK降低了TLR2、MyD88、TAK1、NF-κB和AP-1等相关基因的mRNA表达，也下调了TLR2、MyD88、TAK1、NF-κB和AP-1的蛋白表达。
实验提供了证据表明SK可基于其综合的抗菌和抗炎作用治疗皮肤及软组织感染性疾病。其治疗机制可能与抑制TLR2/MyD88/NF-κB信号通路有关。
