COVID-19患者的结膜聚合酶链反应及其与临床和辅助检查指标的相关性。
Conjunctival polymerase chain reactions in COVID-19 patients and its correlation with clinical and paraclinical indexes.
作者信息
Farzin Maral, Rasoulinejad Seyed Ahmad, Babaei Mansour, Sadeghi Farzin, Sadeghi-Haddad-Zavareh Mahmoud, Firouzjahi Alireza, Heidari Behzad
机构信息
Department of Medicine, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
Department of Ophthalmology, Ayatollah Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran.
出版信息
Iran J Microbiol. 2021 Dec;13(6):757-763. doi: 10.18502/ijm.v13i6.8074.
BACKGROUND AND OBJECTIVES
This study aimed to detect SARS-CoV-2 in conjunctival samples of COVID-19 patients to investigate the transmission route of COVID-19 and its correlation with laboratory indexes.
MATERIALS AND METHODS
In this cross-sectional study, 44 COVID-19 patients were tested for conjunctival PCR in Ayatollah Rouhani hospital of Babol, Iran, in January and February 2021. The conjunctival samples were collected using a conjunctival swab and suspended in a viral transport medium. After RNA extraction and cDNA synthesis, real-time PCR was performed to investigate the SARS-CoV-2 genome in samples. The ocular manifestations and laboratory indexes were evaluated for all patients.
RESULTS
Among 44 COVID-19 patients, 6 samples (13.63%) were positive in terms of conjunctival PCR. The mean ± SD age of conjunctival PCR-positive patients was 76.17 ± 16.61-year-old, while conjunctival PCR-negative COVID-19 patients were aged 57.54 ± 13.61-year-old (p <0.05). D-dimer serum level is significantly higher in conjunctival PCR-positive COVID-19 patients (4001.00 ± 3043.36 μg/ml) compared to normal individuals (496.80 ± 805.92 μg/ml, p <0.01).
CONCLUSION
Our study showed that the conjunctiva and tear contain the SARS-CoV-2 in COVID-19 patients as a possible transmission route.
背景与目的
本研究旨在检测新冠病毒肺炎（COVID-19）患者结膜样本中的严重急性呼吸综合征冠状病毒2（SARS-CoV-2），以调查COVID-19的传播途径及其与实验室指标的相关性。
材料与方法
在这项横断面研究中，于2021年1月和2月在伊朗巴博勒的阿亚图拉·鲁哈尼医院对44例COVID-19患者进行了结膜聚合酶链反应（PCR）检测。使用结膜拭子采集结膜样本，并悬浮于病毒运输培养基中。经过RNA提取和互补DNA（cDNA）合成后，进行实时PCR以研究样本中的SARS-CoV-2基因组。对所有患者的眼部表现和实验室指标进行了评估。
结果
在44例COVID-19患者中，6份样本（13.63%）结膜PCR检测呈阳性。结膜PCR阳性患者的平均年龄±标准差为76.17±16.61岁，而结膜PCR阴性的COVID-19患者年龄为57.54±13.61岁（p<0.05）。与正常个体（496.80±805.92μg/ml，p<0.01）相比，结膜PCR阳性的COVID-19患者的D-二聚体血清水平显著更高（4001.00±3043.36μg/ml）。
结论
我们的研究表明，COVID-19患者的结膜和泪液中含有SARS-CoV-2，这可能是一种传播途径。
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