Yki-Järvinen H, Nikkilä E A, Kubo K, Foley J E
Diabetologia. 1986 May;29(5):287-90. doi: 10.1007/BF00452064.
A method was developed for repeated measurements of glucose transport in human fat cells obtained by needle biopsy aspiration. Assay conditions, reproducibility and normal values of the measurements are described. Transport rates were measured in the absence and presence (25, 50, 100, 200, 800 pmol/l; 8, 80 nmol/l) of insulin using U-(14C)-D-glucose as the tracer. The extracellular glucose concentration was 1.5 mumol/l. The reproducibility of glucose transport measurements was assessed by taking two needle biopsies from opposite sides of the same subject (n = 11). The mean coefficient of variation for maximal glucose transport was 11 +/- 6%. In 14 subjects, a needle biopsy sample was aspirated immediately prior to surgical removal of fat. The maximal insulin-stimulated glucose transport rates averaged 143 +/- 15 and 143 +/- 15 fl/cell X s, and the ED50:s 218 +/- 124 and 160 +/- 28 pmol/l (NS) in fat cells prepared from needle biopsy and surgically removed adipose tissue respectively. The mean coefficient of variation for maximal glucose transport in needle vs. surgical samples was 11 +/- 2%. In 6 subjects, a surgical biopsy was taken twice, with a 1-week interval. The coefficient of variation averaged 9 +/- 2%. We conclude that measurement of glucose transport rates can be done with similar accuracy using fat cells isolated from needle biopsy aspirates and surgically removed adipose tissue. Use of needle biopsy samples permits, however, study of glucose transport in repeat samples of human fat cells, and may therefore be a useful tool for any perturbation studies.
已开发出一种方法，用于对通过针吸活检获取的人体脂肪细胞中的葡萄糖转运进行重复测量。描述了测量的分析条件、可重复性和正常值。使用U-(14C)-D-葡萄糖作为示踪剂，在不存在胰岛素以及存在胰岛素（25、50、100、200、800 pmol/l；8、80 nmol/l）的情况下测量转运速率。细胞外葡萄糖浓度为1.5 μmol/l。通过从同一受试者的相对两侧进行两次针吸活检（n = 11）来评估葡萄糖转运测量的可重复性。最大葡萄糖转运的平均变异系数为11±6%。在14名受试者中，在手术切除脂肪之前立即抽取针吸活检样本。在分别从针吸活检和手术切除的脂肪组织制备的脂肪细胞中，最大胰岛素刺激的葡萄糖转运速率平均分别为143±15和143±15 fl/细胞×秒，ED50分别为218±124和160±28 pmol/l（无显著性差异）。针吸活检样本与手术样本中最大葡萄糖转运的平均变异系数为11±2%。在6名受试者中，进行了两次手术活检，间隔1周。变异系数平均为9±2%。我们得出结论，使用从针吸活检抽吸物和手术切除的脂肪组织中分离的脂肪细胞，可以以相似的准确度进行葡萄糖转运速率的测量。然而，使用针吸活检样本允许对人体脂肪细胞的重复样本进行葡萄糖转运研究，因此可能是任何扰动研究的有用工具。
