Kusuma Sri Agung Fitri, Septyadi Rendy, Sofian Ferry Ferdiansyah
Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, West Java, Indonesia.
J Adv Pharm Technol Res. 2022 Jan-Mar;13(1):7-10. doi: 10.4103/japtr.japtr_286_21. Epub 2022 Jan 21.
This study was intended to discover the natural food preservatives by comparing the antibacterial effect of the ethanolic extract of cinnamon bark, finger root, and moringa leaves toward both the vegetative cells and spores. The antibacterial activities of the investigated extracts were assessed against cells using the agar diffusion method. Whereas the sporicidal test was performed by observing the colony growth, after various times of incubation (1, 3, and 5 h). The investigated extracts produced inhibition in a diameter ranging from 10.6 to 35.3 mm, and it can be classified that the extract of cinnamon bark was the most potent extract to inhibit the vegetative cells form, followed by fingerroot and the moringa leaves extract. Consistently, the ethanolic extract of cinnamon bark and fingerroot significantly yielded sporicidal activities higher than the moringa leaves extract. Both extracts exerted sporicidal activity within 1 h of contact time at the lowest test concentration of 5% w/v, whereas moringa leaves extract required a longer contact time (5 h) at higher concentration of 20% w/v. It can be concluded that cinnamon bark and fingerroot extract have great potential as effective food preservative candidates to inhibit the growth than moringa leaves extract.
本研究旨在通过比较肉桂树皮、莪术根和辣木叶乙醇提取物对营养细胞和孢子的抗菌效果来发现天然食品防腐剂。采用琼脂扩散法评估所研究提取物对细胞的抗菌活性。而杀孢子试验则是通过观察不同孵育时间(1、3和5小时)后的菌落生长来进行的。所研究的提取物产生的抑菌圈直径在10.6至35.3毫米之间,可以分类得出肉桂树皮提取物是抑制营养细胞形态最有效的提取物,其次是莪术根提取物和辣木叶提取物。一致的是,肉桂树皮和莪术根的乙醇提取物产生的杀孢子活性明显高于辣木叶提取物。在最低测试浓度5% w/v下,两种提取物在接触1小时内均表现出杀孢子活性,而辣木叶提取物在20% w/v的较高浓度下需要更长的接触时间(5小时)。可以得出结论,与辣木叶提取物相比,肉桂树皮和莪术根提取物作为有效的食品防腐剂候选物具有更大的抑制生长的潜力。