Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Sciences, Hangzhou, China.
Department of infectious Disease Control and Prevention, HangZhou Center for Disease Control and Prevention, Hangzhou, China.
Front Cell Infect Microbiol. 2022 Feb 10;12:821662. doi: 10.3389/fcimb.2022.821662. eCollection 2022.
Probiotics, prebiotics, and synbiotics can alleviate metabolic syndrome by altering the composition of the gut microbiota. Live combined and has been indicated to promote growth and reduce inflammation in animal models. However, the modulatory effects of live combined R-179 and R-026 (LCBE) on human microbiota remain unclear. The current study examined the growth of these two strains in the presence of various oligosaccharides and assessed the effects of this probiotic mixture on human and murine gut microbiota and . Oligosaccharides improved the growth of R-026 and R-179 as well as increased their production of short-chain fatty acids. R-026 or R-179 co-incubated with and significantly increased the number of the anaerobic bacteria and by fermentation. Moreover, LCBE significantly reduced plasma cholesterol levels in mouse models of hyperlipidemia. LCBE combined with galacto-oligosaccharides led to a significant decrease in the Firmicutes/Bacteroidetes ratio and a significant increase in the relative abundance of and after treating mice with LCBE (0.23 g/day) for eight weeks. Furthermore, fermentation also showed that both the single strains and the two-strain mixture modulated human gut microbiota, resulting in increased and , and decreased . Overall, these results suggest that LCBE can improve host health by reducing the level of cholesterol in mouse models by modifying the composition of the gut microbiota.
益生菌、益生元和合生菌可以通过改变肠道微生物群落的组成来缓解代谢综合征。已经表明，在动物模型中，合生菌（LCBE）可以促进生长并减轻炎症。然而，LCBE 对人类微生物群的调节作用尚不清楚。本研究考察了这两种菌株在各种寡糖存在下的生长情况，并评估了这种益生菌混合物对人类和鼠类肠道微生物群和的影响。寡糖改善了 R-026 和 R-179 的生长，并增加了它们短链脂肪酸的产生。R-026 或 R-179 与 和 共同孵育显著增加了厌氧菌 和 的数量 通过发酵。此外，LCBE 可显著降低高脂血症小鼠模型的血浆胆固醇水平。LCBE 与半乳糖寡糖结合可显著降低厚壁菌门/拟杆菌门的比例，并用 LCBE（0.23 克/天）治疗 8 周后，丰度显著增加 和 ，并减少 。此外，发酵也表明，单一菌株和两菌株混合物均可调节人类肠道微生物群，导致 和 的增加， 和 的减少 。总之，这些结果表明，LCBE 通过改变肠道微生物群落的组成，可以降低小鼠模型中的胆固醇水平，从而改善宿主健康。
