Al-Mariri Ayman, Al-Hallab Laila, Alabras Rasha, Kherbik Heba, Khawajkiah Marwa
Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, Syria.
Clin Exp Vaccine Res. 2022 Jan;11(1):53-62. doi: 10.7774/cevr.2022.11.1.53. Epub 2022 Jan 31.
spp. is a zoonosis that causes undulant fever in humans and abortion in livestock worldwide. Lately, it was conveyed that vaccines developed by irradiation have induced a strong cellular and humoral immune response which have made these types of vaccines highly effective.
In this study, we aimed to use the gamma-irradiated as a vaccine and to study the humoral immune response and cytokines production in order to evaluate it for protecting mice against 544, 16M, and .
The humoral immune response in immunized mice with gamma-irradiated showed a lasting for 8 weeks after immunization. Moreover, immunoglobulin G (IgG), IgG1, IgG2a, and IgG2b isotypes antibodies against were observed after 4 and 8 weeks of the last immunization. It was noticed that the production of tumor necrosis factor-α, interferon-γ, and interleukin (IL)-10 continued after 4 and 8 weeks by splenocytes from immunized BALB/c mice, while no production of IL-4 or IL-5 was observed.
Our results indicate that the protection of BALB/c mice against 16M, 544, and was induced and the developed vaccine at our laboratory could stimulate similar protection to those induced by the traditional vaccine.
[疾病名称]是一种人畜共患病，在全球范围内可导致人类波状热和家畜流产。最近，有报道称经辐射开发的疫苗可诱导强烈的细胞免疫和体液免疫反应，使这些类型的疫苗非常有效。
在本研究中，我们旨在使用经γ射线辐照的[病原体名称]作为疫苗，并研究体液免疫反应和细胞因子产生，以评估其对小鼠抵抗[病原体名称]544、16M和[病原体名称]的保护作用。
用经γ射线辐照的[病原体名称]免疫的小鼠的体液免疫反应在免疫后持续8周。此外，在最后一次免疫4周和8周后观察到针对[病原体名称]的免疫球蛋白G（IgG）、IgG1、IgG2a和IgG2b同种型抗体。注意到免疫的BALB/c小鼠的脾细胞在4周和8周后肿瘤坏死因子-α、干扰素-γ和白细胞介素（IL）-10的产生持续存在，而未观察到IL-4或IL-5的产生。
我们的结果表明，BALB/c小鼠对[病原体名称]16M、544和[病原体名称]的保护作用得以诱导，并且我们实验室开发的疫苗可刺激产生与传统疫苗诱导的保护作用相似的保护作用。
