Protection against virulent spp. by gamma-irradiated in BALB/c mice model.

PURPOSE

spp. is a zoonosis that causes undulant fever in humans and abortion in livestock worldwide. Lately, it was conveyed that vaccines developed by irradiation have induced a strong cellular and humoral immune response which have made these types of vaccines highly effective.

MATERIALS AND METHODS

In this study, we aimed to use the gamma-irradiated as a vaccine and to study the humoral immune response and cytokines production in order to evaluate it for protecting mice against 544, 16M, and .

RESULTS

The humoral immune response in immunized mice with gamma-irradiated showed a lasting for 8 weeks after immunization. Moreover, immunoglobulin G (IgG), IgG1, IgG2a, and IgG2b isotypes antibodies against were observed after 4 and 8 weeks of the last immunization. It was noticed that the production of tumor necrosis factor-α, interferon-γ, and interleukin (IL)-10 continued after 4 and 8 weeks by splenocytes from immunized BALB/c mice, while no production of IL-4 or IL-5 was observed.

CONCLUSION

Our results indicate that the protection of BALB/c mice against 16M, 544, and was induced and the developed vaccine at our laboratory could stimulate similar protection to those induced by the traditional vaccine.