Leng Junke, Dai Xiaofeng, Cheng Xiao, Zhou Hao, Wang Dong, Zhao Jing, Ma Kun, Cui Changhao, Wang Li, Guo Zhaoming
School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin, China.
School of Food and Environment, Dalian University of Technology, Panjin, China.
Front Bioeng Biotechnol. 2022 Feb 9;10:841186. doi: 10.3389/fbioe.2022.841186. eCollection 2022.
Breast cancer is the most common malignant tumor in women. Researchers have found that the combined use of multiple methods to treat tumors is a promising strategy. Here, we have developed a biomimetic nano-platform PDA@MB for tumor targeted photothermal therapy (PTT) combined with chemotherapy. The 4T1 cell membrane loaded with cucurbitacin B (CuB) was used to coat polydopamine (PDA) nanoparticles, which gave PDA@MB nanoparticles the ability to target tumors and escape immune cells from phagocytosis. PDA@MB showed excellent photothermal performance including high photothermal conversion efficiency and photostability, and exhibited outstanding PTT effect under NIR laser irradiation. The high temperature ruptured the PDA@MB membrane to release CuB, which changed the tumor hypoxic environment, down-regulated the FAK/MMP signaling pathway, and significantly inhibited the metastasis and proliferation of tumor cells. The results of experiments indicated that the tumor growth of the 4T1 mouse tumor model was significantly inhibited. Additionally, toxicity studies showed that PDA@MB had good biocompatibility and safety. In conclusion, this study provides a promising chemo-photothermal therapy (CPT) nano-platform for precise and effective breast cancer therapy.
乳腺癌是女性最常见的恶性肿瘤。研究人员发现，联合使用多种方法治疗肿瘤是一种很有前景的策略。在此，我们开发了一种用于肿瘤靶向光热疗法（PTT）联合化疗的仿生纳米平台PDA@MB。负载葫芦素B（CuB）的4T1细胞膜用于包裹聚多巴胺（PDA）纳米颗粒，这赋予了PDA@MB纳米颗粒靶向肿瘤并逃避免疫细胞吞噬的能力。PDA@MB表现出优异的光热性能，包括高光热转换效率和光稳定性，并在近红外激光照射下表现出出色的PTT效果。高温使PDA@MB膜破裂以释放CuB，这改变了肿瘤缺氧环境，下调了FAK/MMP信号通路，并显著抑制了肿瘤细胞的转移和增殖。实验结果表明，4T1小鼠肿瘤模型的肿瘤生长受到显著抑制。此外，毒性研究表明PDA@MB具有良好的生物相容性和安全性。总之，本研究为精确有效的乳腺癌治疗提供了一种很有前景的化学-光热疗法（CPT）纳米平台。
