Department of Pharmacy, The Affiliated Lianyungang Hospital of Xuzhou Medical University/The First People's Hospital of Lianyungang, Lianyungang, China.
Department of Pharmacy, Hainan General Hospital/Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China.
Biomed Res Int. 2022 Feb 18;2022:4294008. doi: 10.1155/2022/4294008. eCollection 2022.
Coronary artery disease (CAD) represents the leading cause of death worldwide. Accumulating evidence also suggests that sirtuins (SIRTS) have been associated with CAD. The present study was aimed at investigating the association between 12 gene polymorphisms for SIRTs and the development of CAD in a Chinese population.
12 SNPs (rs12778366 (T > C), rs3758391 (T > C), rs3740051 (A > G), rs4746720 (C > T), rs7895833 (G > A), rs932658 (A > C) for SIRT1, rs2015 (G > T) for SIRT2, rs28365927 (G > A), rs11246020 (C > T) for SIRT3, rs350844 (G > A), rs350846 (G > C), and rs107251 (C > T) for SIRT6) were selected and assessed in a cohort of 509 CAD patients and 552 matched healthy controls for this study. Genomic DNA from whole blood was extracted, and the SNPs were assessed using MassARRAY method.
TT genotype for rs3758391 and GG genotype for rs7895833 of SIRT1 were at higher risk of CAD, whereas the CC genotype for rs4746720 of SIRT1 was associated with a significantly decreased risk of CAD. The A allele of the rs28365927 of SIRT3 showed a significant decreased risk association with CAD patient group ( = 0.014). Significant difference in genotypes rs350844 (G > A) ( = 0.004), rs350846 (G > C) ( = 0.002), and rs107251 (C > T) ( ≤ 0.01) for SIRT6 was also found between the CAD patients and the healthy controls. Haplotype CTA significantly increased the risk of CAD ( = 0.000118, OR = 1.497, 95%CI = 1.218-1.840), while haplotype GCG significantly decreases the risk of CAD ( = 0.000414, OR = 1.131, 95%CI = 0.791-1.619).
The SNP rs28365927 in the SIRT3 gene and SNP rs350844, rs350846, and rs107251 in the SIRT6 gene present significant associations with CAD in a north Chinese population. Haplotype CTA and GCG generated by rs350846/rs107251/rs350844 in the SIRT6 might also increase and decrease the risk of CAD, respectively.
冠状动脉疾病 (CAD) 是全球范围内导致死亡的主要原因。越来越多的证据表明，沉默调节蛋白 (SIRTs) 与 CAD 有关。本研究旨在调查中国人群中 12 个 SIRTs 基因多态性与 CAD 发展之间的关系。
选择了 12 个 SNP（rs12778366（T > C）、rs3758391（T > C）、rs3740051（A > G）、rs4746720（C > T）、rs7895833（G > A）、rs932658（A > C）用于 SIRT1、rs2015（G > T）用于 SIRT2、rs28365927（G > A）、rs11246020（C > T）用于 SIRT3、rs350844（G > A）、rs350846（G > C）和 rs107251（C > T）用于 SIRT6），并在 509 例 CAD 患者和 552 例匹配的健康对照者中评估了这一队列的 SNP。从全血中提取基因组 DNA，并使用 MassARRAY 方法评估 SNP。
SIRT1 的 rs3758391 的 TT 基因型和 rs7895833 的 GG 基因型患 CAD 的风险较高，而 SIRT1 的 rs4746720 的 CC 基因型与 CAD 显著降低风险相关。SIRT3 的 rs28365927 的 A 等位基因与 CAD 患者组显著降低风险相关（ = 0.014）。SIRT6 的 rs350844（G > A）（ = 0.004）、rs350846（G > C）（ = 0.002）和 rs107251（C > T）（ ≤ 0.01）的基因型也存在显著差异在 CAD 患者和健康对照组之间。CTA 单倍型显著增加 CAD 的风险（ = 0.000118，OR = 1.497，95%CI = 1.218-1.840），而 GCG 单倍型显著降低 CAD 的风险（ = 0.000414，OR = 1.131，95%CI = 0.791-1.619）。
中国北方人群中 SIRT3 基因中的 SNP rs28365927 和 SIRT6 基因中的 SNP rs350844、rs350846 和 rs107251 与 CAD 存在显著关联。SIRT6 中的 rs350846/rs107251/rs350844 产生的 CTA 和 GCG 单倍型也分别可能增加和降低 CAD 的风险。
