Xiong Rui, Hsieh Yi-Wen, Chuang Chiou-Fen
Department of Biological Sciences, University of Illinois at Chicago.
Graduate Program in Neuroscience, University of Illinois at Chicago.
MicroPubl Biol. 2022 Feb 22;2022. doi: 10.17912/micropub.biology.000529. eCollection 2022.
The HMX/NKX MLS-2 transcription factor was previously shown to play sequential roles in AWC general identity and the stochastic AWC/AWC subtype choice during embryogenesis. Here we analyze the expression pattern of endogenous during AWC development using mNeonGreen (mNG) knock-in strains. Similar to transgenic GFP::MLS-2, functional mNG::MLS-2 knock-in displayed nuclear localization in AWC precursor cells but was not observed in AWC during the later embryonic stage. These results suggest that the expression of is below the detectable level and/or the stability of MLS-2 protein is very low in AWC cells.
先前的研究表明，HMX/NKX MLS-2转录因子在胚胎发育过程中，在AWC一般身份和随机的AWC/AWC亚型选择中发挥着相继作用。在这里，我们使用mNeonGreen（mNG）敲入菌株分析了AWC发育过程中内源性[未提及具体物质，原文此处可能有误]的表达模式。与转基因GFP::MLS-2相似，功能性mNG::MLS-2敲入在AWC前体细胞中显示出核定位，但在胚胎后期阶段未在AWC中观察到。这些结果表明，[未提及具体物质，原文此处可能有误]的表达低于可检测水平，和/或MLS-2蛋白在AWC细胞中的稳定性非常低。
