Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
Small. 2022 Apr;18(15):e2106094. doi: 10.1002/smll.202106094. Epub 2022 Feb 27.
This work analyzes the intracellular fate of protein-based nanocarriers along their endolysosomal pathway by means of correlative light and electron microscopy methods. To unambiguously identify the nanocarriers and their degradation remnants in the cellular environment, they are labeled with fluorescent, inorganic nanoplatelets. This allows tracking the nanocarriers on their intracellular pathway by means of electron microscopy imaging. From the present data, it is possible to identify different cell compartments in which the nanocarriers are processed. Finally, three different terminal routes for the intracellular destiny of the nanocarriers are presented. These findings are important to reveal the degradation process of protein nanocapsules and contribute to the understanding of the therapeutic success of an encapsulated drug.
本工作通过相关的光镜和电镜方法分析了蛋白纳米载体在其内体溶酶体途径中的细胞内命运。为了在细胞环境中明确识别纳米载体及其降解残余物，它们用荧光无机纳米板进行标记。这使得可以通过电子显微镜成像来跟踪纳米载体的细胞内途径。根据目前的数据，可以识别纳米载体在其中进行加工的不同细胞区室。最后，提出了纳米载体细胞内命运的三种不同终末途径。这些发现对于揭示蛋白纳米胶囊的降解过程很重要，并有助于理解包封药物的治疗成功。
