201271Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Beograd, Serbia.
Department of Microbiology and Immunology, 186111University of Belgrade Faculty of Pharmacy, Beograd, Serbia.
J Biomater Appl. 2022 May;36(10):1800-1811. doi: 10.1177/08853282211073731. Epub 2022 Feb 27.
Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as . Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones.
耐多药细菌菌株代表了一种新兴的全球健康威胁，也是骨组织工程的一大障碍。骨的细胞外基质的主要成分之一是胶原蛋白，而硒是一种具有抗菌潜力的元素，对骨骼代谢和骨骼健康也很重要。在这里，我们将通过绿色化学还原法合成的硒纳米粒子（SeNPs）掺入胶原蛋白凝胶中，以产生具有抗菌性能的复合材料，即胶原/SeNPs。通过zeta 电位测量、动态光散射电感耦合等离子体质谱（ICP-MS）、X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、光学显微镜、场发射扫描电子显微镜（FE-SEM）和差示扫描量热法对样品进行了全面表征。测试了 SeNPS 以及胶原/SeNPs 对 MRC-5 细胞的细胞毒性。结果表明，胶原/SeNPs 的细胞毒性较低。胶原/SeNPs 对所有测试的革兰氏阳性菌株均表现出显著的抗菌活性，这些菌株是矫形感染的主要病原体。此外，通过成熟的 3D 打印（光刻）方法制造了三维 β-磷酸三钙（3D-TCP）支架，然后用新合成的 SeNPs 或胶原/SeNPs 进行初步涂层。此外，还对未涂层的 3D-TCP 支架以及用胶原/SeNPs 涂层的支架进行了生物膜形成。与未涂层的支架相比，用胶原/SeNPs 涂层的支架上生物膜的产生明显减少。
