Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt.
Biotechnol J. 2022 Feb;17(2):e2100432. doi: 10.1002/biot.202100432. Epub 2021 Nov 21.
BACKGROUND/GOAL/AIM: Plant extract is affordable and does not require any particular conditions; rapid production of nanoparticles using plants offers more advantages than other approaches. Selenium nanoparticles (SeNPs) have received much attention in the last decade due to SeNPs diverse and different applications. Herein, this study aimed to biosynthesize SeNPs using aqueous extract of Urtica dioica leaf through green and ecofriendly method. Moreover to fully characterize SeNPs using different techniques, and to evaluate it for antimicrobial activity as well as anticancer activity.
SeNPs were biosynthesis using aqueous leaf extract of U. dioica (stinging nettle). The biosynthesized SeNPs were characterized using UV-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive electron spectroscopy (EDX), transmission electron microscopy (TEM), and thermal-gravimetric analysis (TGA). Antimicrobial and anticancer activities of biosynthesized SeNPs were assessed. Results illustrated that SeNPs exhibited promising antibacterial activity against Gram-positive and Gram-negative bacteria, as well as unicellular and multi-cellular fungi. Moreover, minimal-inhibitory concentration (MIC) of SeNPs against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus were 250, 31.25, and 500 μg mL , respectively, while were 62.5, 15.62, 31.25, and 7.81 μg mL against Candida albicans, Aspergillus fumigatus, Aspergillus niger, and Aspergillus flavus, respectively. The cytotoxicity of SeNPs was performed on Vero normal-cell line CCL-81, where IC was 173.2 μg mL .
For the first time, aqueous stinging nettle leaf extract was utilized to biosynthesize SeNPs in a green method. SeNPs have outstanding antimicrobial-activity against pathogenic bacterial and fungal strains. Moreover, SeNPs have promising anticancer activity against HepG2 cancerous cell line without cytotoxicity on Vero normal cell line. Finally, the biosynthesized SeNPs via aqueous extract of stinging nettle leaf exhibited potential antibacterial, antifungal, and anticancer action, making them useful in the medical field.
背景/目的/目标：植物提取物价格实惠，且不需要任何特殊条件；与其他方法相比，利用植物快速生产纳米粒子具有更多优势。由于硒纳米粒子（SeNPs）具有多种不同的应用，因此在过去十年中受到了广泛关注。本研究旨在通过绿色环保的方法，利用荨麻叶的水提物来生物合成硒纳米粒子。此外，我们还使用不同的技术对硒纳米粒子进行全面表征，并评估其抗菌活性和抗癌活性。
通过荨麻叶的水提物生物合成了硒纳米粒子。通过紫外-可见分光光度法（UV-Vis）、X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、能谱（EDX）、透射电子显微镜（TEM）和热重分析（TGA）对生物合成的硒纳米粒子进行了表征。评估了生物合成的硒纳米粒子的抗菌和抗癌活性。结果表明，硒纳米粒子对革兰氏阳性和革兰氏阴性细菌以及单细胞和多细胞真菌均表现出良好的抗菌活性。此外，硒纳米粒子对大肠杆菌、铜绿假单胞菌、枯草芽孢杆菌和金黄色葡萄球菌的最小抑菌浓度（MIC）分别为 250、31.25 和 500μg/mL，而对白色念珠菌、烟曲霉、黑曲霉和黄曲霉的 MIC 分别为 62.5、15.62、31.25 和 7.81μg/mL。硒纳米粒子对 Vero 正常细胞系 CCL-81 的细胞毒性为 173.2μg/mL。
首次利用荨麻叶的水提取物以绿色方法生物合成了硒纳米粒子。硒纳米粒子对致病性细菌和真菌菌株具有出色的抗菌活性。此外，硒纳米粒子对 HepG2 癌细胞系具有良好的抗癌活性，对 Vero 正常细胞系无细胞毒性。最后，通过荨麻叶的水提取物生物合成的硒纳米粒子具有潜在的抗菌、抗真菌和抗癌作用，使其在医学领域具有应用价值。
