Institute of Chemistry, Khwaja Fareed University of Engineering and Information Technology, Rahimyar Khan, Pakistan.
Department of food sciences and engineering, Khwaja Fareed University of Engineering and Information Technology, Rahimyar Khan, Pakistan.
World J Microbiol Biotechnol. 2023 Jan 17;39(3):80. doi: 10.1007/s11274-022-03513-z.
Plant mediated synthesis of metallic nanomaterials has emerged as a non-toxic and economical approach to their applications in diverse fields especially in biomedical sciences. Herein, this study first time reporting the use of Bombax ceiba flower extract for synthesis of selenium nanoparticles (SeNPs). Initially, SeNPs were confirmed by turning the color of reaction mixtures from light yellow to brick-red. Scanning electron microscope (SEM) and Transmission electron microscopy (TEM) images showed spherical shaped nanoparticles with smooth surface, size ranges between 30 and 150 nm. Dynamic light scattering (DLS) showed 100-150 nm for the distribution of particle size. X-ray diffraction (XRD) analysis revealed SeNPs crystallinity and confirmed by matching with selenium JCPD card No. 06-362. Energy-dispersive X-ray (EDX) spectra showed presence of pure Se peaks that corroborate the conversion of selenium ions into its elemental form by bio-reduction. Fourier-transform infrared spectroscopy (FTIR) spectra demonstrated that involvement of -OH, C-H, C=C, and C=O functional groups for SeNPs formation. Raman Spectra peaks at 250 cm represent asymmetric trigonal selenium (t-Se). Ultraviolet-visible spectrophotometer (UV-Vis) peaks at 296 and 306 nm which is an indication of surface plasmon resonance (SPR). Moreover, maximum antibacterial activity of SeNPs were observed against Staphylococcus aureus- a gram positive bacteria that possess zone of inhibition (ZOI) 20 mm and Klebsiella pneumonia and Pseudomonas aeruginosa-gram negative bacteria with ZOI 28 mm, respectively, at concentration 100 µg/ml. In addition, the surface functionalities induced through extract components adhere over Se binds with urea and give its detection up to 1mM in milk sample. Conclusively, synthesized SeNPs may function as a potential antibacterial pharmaceutical candidate.
植物介导的金属纳米材料合成作为一种无毒且经济的方法，已经在各个领域得到了广泛的应用，尤其是在生物医学科学领域。在此，本研究首次报道了使用木棉花提取物合成硒纳米颗粒（SeNPs）。首先，通过将反应混合物的颜色从浅黄色变为砖红色，证实了SeNPs 的存在。扫描电子显微镜（SEM）和透射电子显微镜（TEM）图像显示，纳米颗粒呈球形，表面光滑，粒径在 30-150nm 之间。动态光散射（DLS）显示粒径分布在 100-150nm 之间。X 射线衍射（XRD）分析表明，SeNPs 具有结晶度，并通过与硒 JCPD 卡 No.06-362 相匹配得到证实。能谱（EDX）图谱显示存在纯硒峰，这证实了生物还原将硒离子转化为其元素形式。傅里叶变换红外光谱（FTIR）图谱表明，-OH、C-H、C=C 和 C=O 等官能团参与了 SeNPs 的形成。拉曼光谱在 250cm 处的峰代表不对称三角硒（t-Se）。紫外-可见分光光度计（UV-Vis）在 296 和 306nm 处的峰表明存在表面等离子体共振（SPR）。此外，SeNPs 对金黄色葡萄球菌（革兰氏阳性菌）的抑菌活性最强，抑菌圈（ZOI）为 20mm，对肺炎克雷伯菌和铜绿假单胞菌（革兰氏阴性菌）的抑菌活性分别为 28mm，浓度为 100μg/ml。此外，提取物成分诱导的表面官能团与 Se 结合，并与尿素结合，在牛奶样品中检测到高达 1mM 的 Se。综上所述，合成的 SeNPs 可能具有作为潜在抗菌药物的潜力。
