Department of Hepatitis B Vaccine Production, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran.
Department of Chemical Engineering, Faculty of Engineering, Arak University, Arak, Iran.
Prep Biochem Biotechnol. 2023;53(1):31-39. doi: 10.1080/10826068.2022.2035746. Epub 2022 Feb 28.
Various industries ranging from water purification to pharmaceutical production have experienced multi separation steps that impose more process time and contamination possibility by batch operation. We propose a developed microfluidic particle sorter (miniaturized hydrocyclone) that adopts centrifugal force as it has ability to decline the number of separation steps and the risk of extrinsic contamination in continuous process. While biological industries have not relied on mini hydrocyclones considerably because of low efficiency and microfabrication difficulties, current work has been planned to conquer these obstacles. In this research, biomass separation from fermentation broth by 3 mm hydrocyclones was investigated. The effect of apex size, feed flow rate, hydrocyclone geometry were analyzed numerically in four mini-hydrocyclones. The most efficient mini-hydrocyclone was chosen to be made by elegant additive manufacturing technology and studied experimentally. The separation efficiency was achieved up to 90% while the concentration ratio of heavy stream (apex) to dilute stream (vortex finder) was reached more than twofold. The mini hydrocyclone performance in view of energy target was studied by Euler-Reynolds-Efficiency plots. The 4 μm cut size was achieved that is promising high throughput separation for biological particles.
从水净化到制药生产等各种行业都经历了多次分离步骤，分批操作会增加工艺时间和污染的可能性。我们提出了一种经过改进的微流粒子分类器（微型水力旋流器），它采用离心力作为推动力，具有减少分离步骤数量和降低连续过程中外部污染风险的能力。虽然生物产业由于效率低和微加工困难而没有大量依赖微型水力旋流器，但目前已经计划克服这些障碍。在这项研究中，通过 3mm 水力旋流器从发酵液中分离生物质。在四个微型水力旋流器中，数值分析了顶点尺寸、进料流量、水力旋流器几何形状的影响。选择最有效的微型水力旋流器通过优雅的添加剂制造技术制造，并进行了实验研究。分离效率高达 90%，而重流（顶点）与稀流（涡流探测器）的浓度比超过两倍。通过 Euler-Reynolds-Efficiency 图研究了微型水力旋流器在能量指标方面的性能。实现了 4μm 的切割尺寸，这对于生物颗粒的高通量分离具有广阔的前景。
