Furness D N, Hackney C M
Hear Res. 1986;21(3):243-9. doi: 10.1016/0378-5955(86)90222-4.
Further observations on the detailed morphology of stereocilia have been made using high-resolution scanning-electron microscopy of osmium-thiocarbohydrazide-coated guinea pig cochleae. Three types of cross-link have been observed between stereocilia. Side-to-side and row-to-row linkages are composed of a filamentous network whilst upward-pointing links are a fine single strand, often with a terminal widening. The stereocilia have rough surfaces. These features are observed on both inner and outer hair cells despite reported sensitivity to long periods of osmium fixation. We suggest that osmium sensitivity may be altered by the buffering conditions used during preparation. The observations on osmium-coated material correspond more closely with those made using transmission-electron microscopy than those made using other scanning-electron microscopical preparation techniques, since gold-coating artefacts are absent and the degree of specimen collapse is less. This has enabled us to observe fine details of the links and their attachments which have not been reported previously in SEM.
利用经锇-硫代碳酰肼处理的豚鼠耳蜗的高分辨率扫描电子显微镜，对静纤毛的详细形态进行了进一步观察。在静纤毛之间观察到三种类型的交联。侧向和排与排之间的连接由丝状网络组成，而向上的连接是细单链，通常末端变宽。静纤毛表面粗糙。尽管有报道称对内耳和外毛细胞长期进行锇固定敏感，但在这两种细胞上都观察到了这些特征。我们认为，制备过程中使用的缓冲条件可能会改变对锇的敏感性。与使用其他扫描电子显微镜制备技术相比，对经锇处理材料的观察结果与使用透射电子显微镜的观察结果更接近，因为不存在金涂层假象，且样本塌陷程度较小。这使我们能够观察到连接及其附着的精细细节，这些细节在扫描电子显微镜中此前尚未有报道。
