Ali Sameh S, Abd Elnabi Manar K, Alkherkhisy Mohammad M, Hasan Abdulkarim, Li Fanghua, Khalil Maha, Sun Jianzhong, El-Zawawy Nessma
Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
J Appl Biomed. 2022 Feb 16. doi: 10.32725/jab.2022.003.
Thirty-one of sixty dyspeptic patients tested positive for Helicobacter pylori colonization in this study, as determined by histopathology and 16S rRNA. The cytotoxin-associated gene A (cagA) and vacuolating cytotoxin A (vacA) genes were found in 67.7 and 93.5% of H. pylori patients, respectively. The cagA gene was found to be associated with 100% of patients with duodenal erosion and ulceration identified via endoscopy examination. In addition, 86.7% of patients with cancerous and precancerous lesions, glandular atrophy, and intestinal metaplasia identified via histopathology examination. The vacA s1m1 mutation was associated with more severe forms of gastric erosion and ulceration, as well as the presence of precancerous and cancerous lesions. Eighteen (64.3%) of the twenty-eight isolates were classified as multi-drug resistant (MDR) or pan-drug resistant (PDR) H. pylori. Due to a resurgence of interest in alternative therapies derived from plants as a result of H. pylori resistance to the majority of commonly used antibiotics, the inhibitory activity of five essential oils extracted from some commonly used medicinal plants was evaluated in vitro against drug-resistant H. pylori clinical isolates. Cinnamomum zeylanicum essential oil demonstrated the highest anti-H. pylori activity when compared to the other essential oils tested. Cinnamaldehyde was the most abundant compound in C. zeylanicum (65.91%). The toxicological evaluation established the safety of C. zeylanicum oil for human use. As a result, C. zeylanicum essential oil may represent a novel antibacterial agent capable of combating drug-resistant H. pylori carrying cytotoxin genes.
在本研究中，60名消化不良患者中有31名经组织病理学和16S rRNA检测显示幽门螺杆菌定植呈阳性。细胞毒素相关基因A（cagA）和空泡毒素A（vacA）基因分别在67.7%和93.5%的幽门螺杆菌患者中被发现。通过内镜检查发现，cagA基因与100%的十二指肠糜烂和溃疡患者相关。此外，通过组织病理学检查发现，86.7%的患者存在癌性和癌前病变、腺体萎缩和肠化生。vacA s1m1突变与更严重的胃糜烂和溃疡形式以及癌前和癌性病变的存在有关。28株分离株中有18株（64.3%）被归类为耐多药（MDR）或泛耐药（PDR）幽门螺杆菌。由于幽门螺杆菌对大多数常用抗生素产生耐药性，人们对源自植物的替代疗法的兴趣再度兴起，因此对从一些常用药用植物中提取的五种精油对耐药幽门螺杆菌临床分离株的体外抑制活性进行了评估。与其他测试精油相比，锡兰肉桂精油表现出最高的抗幽门螺杆菌活性。肉桂醛是锡兰肉桂中含量最丰富的化合物（65.91%）。毒理学评估确定了锡兰肉桂油对人体使用的安全性。因此，锡兰肉桂精油可能是一种新型抗菌剂，能够对抗携带细胞毒素基因的耐药幽门螺杆菌。
