() plays a crucial role in the pathogenesis of gastritis, peptic ulcer, and gastric cancer. The presence of pathogenicity islands (PAI) genes contributes to the pathogenesis of many gastrointestinal disorders. Cytotoxin-associated gene A () and vacuolating cytotoxin gene () are the most known virulence genes in . So, our aim was to study virulence genes' role in gastric disorders pathogenesis. Our study included 150 adult patients who suffered dyspeptic symptoms and were referred to the GIT endoscopy unit. Gastric biopsies were attained for rapid urease test (RUT) and histopathological examination, and multiplex PCR technique for detection of virulence genes was performed. It was found that 100 specimens were (RUT) positive, of which sixty samples (60%) were PCR positive for gene. The and genes were identified in 61.6% and 53% of strains, respectively. Only 5 cases were -positive and -negative. The most virulent s1 allele existed in 56.6% of cases. Out of the 60 strains, 66% had at least one virulence gene and 34% did not show any virulence gene. infection showed significant increase with age. are prevalent amid dyspeptic patients in our region. The main genotype combinations were +/+ of genotype and they were frequently associated with peptic ulcer diseases, gastritis, and gastroesophageal reflux disease.