State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, Zhejiang 311300, China.
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, Zhejiang 311300, China.
Food Chem. 2022 Aug 1;384:132454. doi: 10.1016/j.foodchem.2022.132454. Epub 2022 Feb 14.
The seeds of Torreya grandis are necessary to go through a ripening process, which eventually leads to nutrition conversion and the production of edible nuts. However, the molecular basis of nutrition conversion remains unclear. Here, transcriptome sequencing was performed on seeds treated with different temperature and humidity. A total of 881 unigenes related to nutrition conversion were identified. The correlations between nutrient content and gene expression suggested that sucrose phosphate synthase (SPS), dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (DLST), glycerol-3-phosphate acyltransferase (GPAT) and Pyruvate kinase (PK) may play key roles in nutrition conversion. Transient over-expression of TgDLST, TgPK and TgGPAT in tobacco leaves promoted nutritional conversion. Moreover, enzyme activity analysis indicated that diacylglycerol acyltransferase (DGAT) and pyruvate dehydrogenase (PDH) activities may also accelerate the nutritional conversion. This study uncovers the molecular basis of nutrition conversion in T. grandis seeds, which critical for shortening the time of nutrition conversion.
香榧种子需要经过成熟过程，最终导致营养物质转化和可食用坚果的产生。然而，营养物质转化的分子基础仍不清楚。在这里，对用不同温度和湿度处理的种子进行了转录组测序。总共鉴定出了 881 个与营养物质转化相关的基因。营养物质含量与基因表达的相关性表明，蔗糖磷酸合酶（SPS）、二氢硫辛酸赖氨酸残基琥珀酰基转移酶 2-酮戊二酸脱氢酶复合物（DLST）、甘油-3-磷酸酰基转移酶（GPAT）和丙酮酸激酶（PK）可能在营养物质转化中发挥关键作用。在烟草叶片中转瞬时过表达 TgDLST、TgPK 和 TgGPAT 促进了营养物质的转化。此外，酶活性分析表明，二酰基甘油酰基转移酶（DGAT）和丙酮酸脱氢酶（PDH）的活性也可能加速营养物质的转化。本研究揭示了香榧种子营养物质转化的分子基础，这对于缩短营养物质转化时间至关重要。
