Bhinge Somnath D, Jadhav Namdeo R, Randive Dheeraj S, Bhutkar Mangesh A, Chavan Rohankumar, Kumbhar Bajarang V
Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Maharashtra, 415 404, India.
Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, Maharashtra, 416 013, India.
J Ayurveda Integr Med. 2022 Apr-Jun;13(2):100542. doi: 10.1016/j.jaim.2022.100542. Epub 2022 Feb 25.
In Aayurveda, Blumea eriantha DC has been used in the management of various diseases and is found to exhibit antioxidant and anti-hyperlipidemic, hypoglycemic, anti-diarrhoeal, larvicidal, antimicrobial properties.
The present study has focused on isolation of the active fraction from B. eriantha DC extract and to investigate its effect as a hair growth promoter along with identification of phytoconstituent(s) responsible for hair growth activity and its probable mechanism of action.
Our work introduces an effective isolation protocol for the active fraction from B. eriantha DC extract using chromatographic techniques. Fraction A was isolated by using mobile phase toluene:acetone (9:1). In-vitro and in-vivo methods were executed for the evaluation of hair growth activity. Moreover, the docked conformations of the isolated phytoconstituent Dimethyl sulfone was compared to Minoxidil for selected proteins namely 2FGF, 2PVC and 4U7P. The PDB identifications 2PVC (DNMT3L recognizes unmethylated histone H3 lysine 4), 4U7P (Crystal structure of DNMT3A-DNMT3L complex and 2FGF (Human Basic Fibroblast Growth Factor) were downloaded from Protein Data Bank.
The study data revealed that B. eriantha DC alcoholic extracts exhibited prominent hair growth activity and it was affirmed that Dimethyl sulfone a phyto-constituent isolated from B. eriantha DC alcoholic extract contributed for the same.
The findings strongly suggest hair growth promotion potential of the extract of B. eriantha DC.
在阿育吠陀医学中，艾纳香已被用于治疗各种疾病，并被发现具有抗氧化、抗高血脂、降血糖、止泻、杀幼虫和抗菌特性。
本研究着重于从艾纳香提取物中分离活性成分，并研究其作为头发生长促进剂的作用，同时鉴定对头发生长活性负责的植物成分及其可能的作用机制。
我们的工作介绍了一种使用色谱技术从艾纳香提取物中分离活性成分的有效方案。通过使用甲苯：丙酮（9:1）的流动相分离出组分A。采用体外和体内方法对头发生长活性进行评估。此外，将分离出的植物成分二甲基砜与米诺地尔针对选定的蛋白质2FGF、2PVC和4U7P的对接构象进行了比较。PDB识别号2PVC（DNMT3L识别未甲基化的组蛋白H3赖氨酸4）、4U7P（DNMT3A-DNMT3L复合物的晶体结构）和2FGF（人碱性成纤维细胞生长因子）从蛋白质数据库下载。
研究数据显示，艾纳香乙醇提取物表现出显著的头发生长活性，并且证实从艾纳香乙醇提取物中分离出的植物成分二甲基砜对此有贡献。
这些发现有力地表明了艾纳香提取物具有促进头发生长的潜力。
