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[应用免疫荧光抗体反应对牛带绦虫囊尾蚴病进行活体诊断]

[Intravital diagnosis of saginata cysticercosis of cattle by means of the immunofluorescent antibody reaction].

作者信息

Flentje B, Buchwalder R, Hiepe T

出版信息

Arch Exp Veterinarmed. 1978;32(2):205-12.

PMID:352301
Abstract

Investigations in intravital diagnostics of fin infestation in cattle were performed in 3 month aged cattle experimentally infected by different doses of Taeniarhynchus-saginatus-eggs by means of immunofluorescent antibody technique using a standardized antigen. Regardless of the amount of the infection dose these occured already 2 weeks after infection positive antibody values of at least 1:40 reaching the maximum value of 1:640 4-6 weeks after infection (in cases of strong fin infestation) and 1:160, respectively (in cases of low fin infestation) and remaining positively up to the termination of experiments (23 and 18 weeks, respectively post infectionem). Negative reactions were not observed. With regard to the economy this method is superior to other immundiagnostical procedures hitherto used with success in detection of Cysticercus bovis.

摘要

采用标准化抗原,通过免疫荧光抗体技术,对3月龄经不同剂量牛带绦虫虫卵实验感染的牛进行了鳍部寄生虫活体诊断研究。无论感染剂量多少,感染后2周抗体值即呈阳性,至少为1:40,感染后4 - 6周(鳍部严重感染的情况)分别达到最大值1:640,(鳍部轻度感染的情况)为1:160,直至实验结束(分别在感染后23周和18周)抗体一直呈阳性。未观察到阴性反应。就经济性而言,该方法优于迄今成功用于检测牛囊尾蚴的其他免疫诊断方法。

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