Biogenesi Reproductive Medicine Centre - Eugin Group, Istituti Clinici Zucchi, Monza, Italy; and Department of Structural and Functional Biology, Sao Paulo State University, Botucatu, Brazil.
Department of Structural and Functional Biology, Sao Paulo State University, Botucatu, Brazil.
Reprod Fertil Dev. 2021 Dec;34(2):27-35. doi: 10.1071/RD21278.
In vitro maturation (IVM) has been applied in numerous different contexts and strategies in humans and animals, but in both cases it represents a challenge still far from being overcome. Despite the large dataset produced over the last two decades on the mechanisms that govern antral follicular development and oocyte metabolism and differentiation, IVM outcomes are still unsatisfactory. This review specifically focuses on data concerning the potential consequences of using supraphysiological levels of FSH during IVM, as well as on the regulation of oocyte chromatin dynamics and its utility as a potential marker of oocyte developmental competence. Taken together, the data revisited herein indicate that a significant improvement in IVM efficacy may be provided by the integration of pre-OPU patient-specific protocols preparing the oocyte population for IVM and more physiological culture systems mimicking more precisely the follicular environment that would be experienced by the recovered oocytes until completion of metaphase II.
体外成熟 (IVM) 在人类和动物的许多不同情况下和策略中都有应用，但在这两种情况下，它仍然代表着一个远未被克服的挑战。尽管在过去的二十年中产生了大量关于窦卵泡发育和卵母细胞代谢和分化所遵循的机制的数据，但 IVM 的结果仍然不尽如人意。本综述特别关注有关在 IVM 期间使用超生理水平的 FSH 的潜在后果的数据，以及卵母细胞染色质动力学的调节及其作为卵母细胞发育能力的潜在标志物的用途。综上所述，本文中回顾的数据表明，通过整合预先 OPU 患者特异性方案，为 IVM 准备卵母细胞群体，并使用更接近卵泡环境的更生理的培养系统来模拟恢复的卵母细胞在完成第二次减数分裂中期之前将经历的环境，IVM 的效果可能会得到显著改善。
