College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China.
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 May 15;273:121075. doi: 10.1016/j.saa.2022.121075. Epub 2022 Feb 23.
Hypochlorous acid (HOCl), one of the most important ROS in living organisms, appears to serve an important role in the immune system in vivo. Endoplasmic reticulum (ER), the largest organelle in cells, manages many biological processes connected to vital activities. To better obtain insight into the relationship of ER stress and HOCl level, a ratiometric fluorescent probe RHE, based on rhodamine combined with HBT and ER-targeting group, was designed and synthesized for HOCl detection in the ER. Probe RHE shows a large stokes shift about 155 nm, which is derived to ESIPT principle. In addition, probe RHE exhibited excellent properties such as fast response (<80 s), high sensitivity with a low detection limit (40 nM), high selectivity and anti-interference. Moreover, probe RHE displayed an excellent ER-targeting ability and had been successfully applied for detection of exogenous and endogenous HOCl in HepG2 cells.
次氯酸（HOCl）是生物体内最重要的活性氧（ROS）之一，似乎在体内免疫系统中发挥着重要作用。内质网（ER）是细胞中最大的细胞器，管理着许多与生命活动相关的生物过程。为了更好地了解内质网应激和 HOCl 水平之间的关系，设计并合成了基于罗丹明与 HBT 结合并带有内质网靶向基团的比率型荧光探针 RHE，用于检测 ER 中的 HOCl。探针 RHE 表现出约 155nm 的大斯托克斯位移，这归因于 ESIPT 原理。此外，探针 RHE 还表现出快速响应（<80s）、高灵敏度（检测限低至 40nM）、高选择性和抗干扰性等优异性能。此外，探针 RHE 显示出优异的内质网靶向能力，并已成功应用于 HepG2 细胞中外源和内源性 HOCl 的检测。
