Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests, Hainan University, School of Plant Protection, Ministry of Education, Haikou, Hainan, 570228, PR China.
Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Hainan University, Ministry of Education, Haikou, Hainan, 570228, PR China.
BMC Microbiol. 2022 Mar 1;22(1):67. doi: 10.1186/s12866-022-02479-0.
Salt stress threaten the growth of plants, and even aggravate plant disease. In this article, salt-tolerant Trichoderma strain was isolated, and its potential to alleviate salt stress and diminish cucumber root rot caused by Fusarium oxysporum was evaluated.
Twenty-seven Trichoderma isolates were isolated from samples of sea muds and algae collected from the South Sea of China. Among these, the isolate HN082102.1 showed the most excellent salt tolerance and antagonistic activity against F. oxysporum causing root rot in cucumber and was identified as T. atroviride. Its antagonism ability may be due to mycoparasitism and inhibition effect of volatile substances. The application of Trichoderma mitigated the adverse effects of salt stress and promoted the growth of cucumber under 100 mM and 200 mM NaCl, especially for the root. When T. atroviride HN082102.1 was applied, root fresh weights increased by 92.55 and 84.86%, respectively, and root dry weights increased by 75.71 and 53.31%, respectively. Meanwhile, the application of HN082102.1 reduced the disease index of cucumber root rot by 63.64 and 71.01% under 100- and 0-mM saline conditions, respectively, indicating that this isolate could inhibit cucumber root rot under salt stress.
This is the first report of salt-tolerant T. atroviride isolated from marine habitat showing antagonistic activity to F. oxysporum, and the results provide evidence for the novel strain T. atroviride HN082102.1 in alleviating salt stress and diminishing cucumber root rot, indicating that T. atroviride strain HN082102.1 can be used as biological control agent in saline alkali land.
盐胁迫威胁植物的生长，甚至加重植物病害。本文从南海采集的海泥和海藻样本中分离出耐盐性的木霉菌株，并评估其缓解盐胁迫和减轻黄瓜枯萎病的潜力。
从中国南海采集的海泥和海藻样本中分离出 27 株木霉。其中，HN082102.1 菌株对盐胁迫的耐受性和对引起黄瓜根腐病的尖孢镰刀菌的拮抗活性最高，被鉴定为深绿木霉。其拮抗能力可能是由于其对尖孢镰刀菌的寄生和挥发性物质的抑制作用。木霉的应用减轻了盐胁迫对黄瓜的不良影响，并促进了 100mM 和 200mM NaCl 下黄瓜的生长，特别是对根的生长。当应用深绿木霉 HN082102.1 时，根鲜重分别增加了 92.55%和 84.86%，根干重分别增加了 75.71%和 53.31%。同时，HN082102.1 在 100mM 和 0mM 盐胁迫条件下，分别降低了黄瓜根腐病的病指 63.64%和 71.01%，表明该分离物可抑制盐胁迫下的黄瓜根腐病。
这是首次从海洋生境中分离出具有拮抗尖孢镰刀菌活性的耐盐性深绿木霉的报道，结果为耐盐性深绿木霉 HN082102.1 缓解盐胁迫和减轻黄瓜根腐病提供了证据，表明深绿木霉 HN082102.1 菌株可作为盐碱地的生物防治剂。
