IPICYT, División de Biología Molecular, Laboratorio de Genómica Funcional y Comparativa, Camino a la Presa San José 2055. Col. Lomas 4 Sección, 78216, San Luis Potosí, SLP, Mexico.
IPICYT, CONAHCYT, Centro Nacional de Supercomputo, Laboratorio de Inteligencia Artificial y Bioinformática, Camino a la Presa San José 2055. Col. Lomas 4 sección, 78216, San Luis Potosí, SLP, Mexico.
Sci Rep. 2024 Jan 30;14(1):2466. doi: 10.1038/s41598-023-48332-w.
Fungi of the Trichoderma genus are called "biostimulants" because they promote plant growth and development and induce disease resistance. We used conventional transcriptome and gene co-expression analyses to understand the molecular response of the plant Arabidopsis thaliana to inoculation with Trichoderma atroviride or Trichoderma virens. The transcriptional landscape of the plant during the interaction with these fungi showed a reduction in functions such as reactive oxygen species production, defense mechanisms against pathogens, and hormone signaling. T. virens, as opposed to T. atroviride, was more effective at downregulating genes related to terpenoid metabolism, root development, and chemical homeostasis. Through gene co-expression analysis, we found functional gene modules that closely link plant defense with hypoxia. Notably, we found a transcription factor (locus AT2G47520) with two functional domains of interest: a DNA-binding domain and an N-terminal cysteine needed for protein stability under hypoxia. We hypothesize that the transcription factor can bind to the promoter sequence of the GCC-box that is connected to pathogenesis by positioned weight matrix analysis.
木霉属真菌被称为“生物刺激素”，因为它们能促进植物的生长和发育，并诱导植物的抗病性。我们采用常规的转录组和基因共表达分析，来理解拟南芥在接种深绿木霉或绿木霉时的分子反应。在与这些真菌相互作用的过程中，植物的转录组图谱显示，与活性氧产生、防御病原体的机制以及激素信号传导等功能相关的基因表达下调。与深绿木霉相比，绿木霉更有效地下调与萜类代谢、根系发育和化学平衡相关的基因。通过基因共表达分析，我们发现了与植物防御和缺氧密切相关的功能基因模块。值得注意的是，我们发现了一个转录因子（AT2G47520 基因），它具有两个感兴趣的功能域：一个 DNA 结合域和一个 N 端半胱氨酸，在缺氧条件下该半胱氨酸对于维持蛋白稳定性是必需的。我们假设，通过位置权重矩阵分析，该转录因子可以结合到与发病机制相关的 GCC 框的启动子序列上。
