Wellcome Centre for Mitochondrial Research, Medical School, Newcastle University, Newcastle-upon-Tyne, NE2 4HH, UK.
Biosciences Institute, Medical School, Newcastle University, Newcastle-upon-Tyne, NE2 4HH, UK.
Anal Bioanal Chem. 2022 Jul;414(18):5483-5492. doi: 10.1007/s00216-022-03944-5. Epub 2022 Mar 2.
Intracellular heterogeneity contributes significantly to cellular physiology and, in a number of debilitating diseases, cellular pathophysiology. This is greatly influenced by distinct organelle populations and to understand the aetiology of disease, it is important to have tools able to isolate and differentially analyse organelles from precise location within tissues. Here, we report the development of a subcellular biopsy technology that facilitates the isolation of organelles, such as mitochondria, from human tissue. We compared the subcellular biopsy technology to laser capture microdissection (LCM) that is the state-of-the-art technique for the isolation of cells from their surrounding tissues. We demonstrate an operational limit of >20 µm for LCM and then, for the first time in human tissue, show that subcellular biopsy can be used to isolate mitochondria beyond this limit.
细胞内异质性对细胞生理学有重要贡献，在许多使人虚弱的疾病中，对细胞病理生理学也有重要贡献。这在很大程度上受到不同细胞器群体的影响，为了了解疾病的病因，拥有能够从组织内特定位置分离和差异分析细胞器的工具非常重要。在这里，我们报告了一种亚细胞活检技术的发展，该技术有助于从人体组织中分离细胞器，如线粒体。我们将亚细胞活检技术与激光捕获显微切割（LCM）进行了比较，LCM 是从周围组织中分离细胞的最先进技术。我们证明了 LCM 的操作极限大于 20μm，然后首次在人体组织中表明，亚细胞活检可以用于分离超过此极限的线粒体。
