Department of ENT, North Eastern Indira Gandhi Regional Institute of Health & Medical Sciences (NEIGRIHMS), Shillong, India.
Department of Pharmacology (Animal House), North Eastern Indira Gandhi Regional Institute of Health & Medical Sciences (NEIGRIHMS), Shillong, India.
Animal Model Exp Med. 2022 Apr;5(2):183-188. doi: 10.1002/ame2.12198. Epub 2022 Jan 18.
To formulate an anesthesia protocol for safe and satisfactory anesthesia for ear surgery in rats.
The rats were anesthetized with xylazine (10 mg/kg body weight) and ketamine at doses of 80, 50, 40, and 30 mg/kg body weight or with isoflurane anesthesia (2%-3.5% in 100% oxygen; maintenance dose 1.5%-3.5%). The anesthesia induction, surgery, and recovery time were recorded.
In total, 17 rats were induced by varying doses of ketamine-xylazine and 28 rats with isoflurane. Mean induction time with ketamine-xylazine was 6 ± 2.9 min compared with 3.8 ± 1.1 min with isoflurane. Mean recovery time with ketamine-xylazine was 142.6 ± 49.3 min compared with 4.1 ± 1.2 min with isoflurane. A mortality of 4 animals after developing dyspnea was recorded with ketamine-xylazine.
Isoflurane anesthesia offers appropriate induction and recovery times and low mortality rates for the surgeries performed. Isoflurane anesthesia offers reliable results for ear surgery in rats. However, more equipment and technical skills are needed.
制定一个安全且令人满意的大鼠耳部手术麻醉方案。
大鼠用盐酸二甲噻嗪（10mg/kg 体重）和盐酸氯胺酮以 80、50、40 和 30mg/kg 体重剂量麻醉，或用异氟烷麻醉（100%氧气中 2%-3.5%；维持剂量 1.5%-3.5%）。记录麻醉诱导、手术和恢复时间。
总共 17 只大鼠用不同剂量的氯胺酮-二甲噻嗪诱导，28 只大鼠用异氟烷诱导。用氯胺酮-二甲噻嗪诱导的平均时间为 6±2.9 分钟，而用异氟烷的平均时间为 3.8±1.1 分钟。用氯胺酮-二甲噻嗪恢复的平均时间为 142.6±49.3 分钟，而用异氟烷的平均时间为 4.1±1.2 分钟。用氯胺酮-二甲噻嗪诱导时，有 4 只动物出现呼吸困难，导致死亡。
异氟烷麻醉具有适当的诱导和恢复时间，且死亡率低，适用于所进行的手术。异氟烷麻醉为大鼠耳部手术提供了可靠的结果。但是，需要更多的设备和技术技能。
