[三萜类生物合成中的氧化角鲨烯环化酶：综述]

[Oxidosqualene cyclases in triterpenoids biosynthesis: a review].

作者信息

Chen Cuiyu, Pang Yaru, Chen Quanbing, Li Chun, Lü Bo

机构信息

Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 102488, China.

Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2022 Feb 25;38(2):443-459. doi: 10.13345/j.cjb.210169.

DOI:10.13345/j.cjb.210169

PMID:35234375

Abstract

Triterpenoids are one of the most diverse compounds in plant metabolites, and they have a wide variety of physiological activities and are of important economic value. Oxidosqualene cyclases catalyze the cyclization of 2, 3-oxidosqualene to generate different types of sterols and plant triterpenoids, which is of great significance to the structural diversity of natural products. However, the mechanism of the diversified cyclization of 2, 3-oxidosqualene catalyzed by oxidosqualene cyclases remains unclear. This review summarized the research progress of oxidosqualene cyclases from the aspects of catalytic function, molecular evolutionary relationship between genes and proteins, protein structure, molecular simulation and molecular calculations, which may provide a reference for protein engineering and metabolic engineering of triterpene cyclase.

摘要

三萜类化合物是植物代谢产物中种类最为多样的化合物之一，它们具有广泛的生理活性且具有重要的经济价值。氧化鲨烯环化酶催化2,3-氧化鲨烯环化生成不同类型的甾醇和植物三萜类化合物，这对天然产物的结构多样性具有重要意义。然而，氧化鲨烯环化酶催化2,3-氧化鲨烯多样化环化的机制仍不清楚。本文综述了氧化鲨烯环化酶在催化功能、基因与蛋白质的分子进化关系、蛋白质结构、分子模拟和分子计算等方面的研究进展，可为三萜环化酶的蛋白质工程和代谢工程提供参考。