Hunan Provincial Key Laboratory of Dong Medicine, Ethnic Medicine Research Center, Hunan University of Medicine, Huaihua 418000, China.
Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, School of Pharmacy, Hunan University of Medicine, Huaihua 418000, China.
Molecules. 2023 Mar 20;28(6):2782. doi: 10.3390/molecules28062782.
The tuberous roots of (Pan) are an edible and medicinal resource in Qinghai-Tibetan Plateau, China. The triterpenoids from tuberous roots have shown promising anti-cancer, hepatoprotective, and anti-inflammatory properties. In this study, we carried out phylogenetic analysis of squalene synthases (), squalene epoxidases (), and oxidosqualene cyclases () in the pathway of triterpenes. In total, 6, 26, and 20 genes of , , and were retrieved from the genome of Pan, respectively. Moreover, 6 and 25 genes expressed in two sub-genomes (A and B) of Pan. were not expanded after whole-genome duplication (WGD), and the duplicated genes were detected in . Twenty were divided into two clades of cycloartenol synthases (CASs) and -amyrin synthases (-ASs) by a phylogenetic tree, characterized with gene duplication and evolutionary divergence. We speculated that -ASs and CASs may participate in triterpenes synthesis. The data presented act as valuable references for future studies on the triterpene synthetic pathway of Pan.
（Pan）的块根是中国青藏高原地区的一种食用和药用资源。块根中的三萜类化合物具有有希望的抗癌、保肝和抗炎特性。在这项研究中，我们对三萜类化合物生物合成途径中的鲨烯合酶（）、鲨烯环氧化酶（）和角鲨烯环化酶（）进行了系统发育分析。总共从 Pan 的基因组中分别检索到 6、26 和 20 个 、 和 基因。此外，在 Pan 的两个亚基因组（A 和 B）中表达了 6 个 和 25 个 。在全基因组复制（WGD）后未发生扩张，并且在 中检测到了重复基因。通过系统发育树将 20 个 分为角鲨烯合酶（CASs）和 - 齐墩果烷合酶（-ASs）两个分支，其特征是基因重复和进化分歧。我们推测 -ASs 和 CASs 可能参与三萜类化合物的合成。所提供的数据为未来研究 Pan 的三萜类化合物合成途径提供了有价值的参考。
