Bjøro K, Hovig T, Stokke K T, Stray-Pedersen S
Prostaglandins. 1986 Apr;31(4):683-98. doi: 10.1016/0090-6980(86)90174-7.
Four major prostanoids (6-keto-PGF1 alpha, PGE2, PGF2 alpha and TXB2) were measured by specific radioimmunoassays in the outputs from human umbilical vessels perfused in vitro. As evaluated by scanning electron microscopy (SEM) only few blood platelets were attached to the vessel wall. After an initial flush with decreasing concentrations of all four prostanoids, a stable stage was reached, lasting for 4-5 hours. During this stage the production could be inhibited by indomethacin and only slightly stimulated with arachidonic acid. The TXA2 synthetase inhibitor UK 38485 depressed the TXB2 production, while only slightly affecting the other three prostanoids at very high concentrations. The arteries produced relatively more 6-keto-PGF1 alpha than did the vein.
采用特异性放射免疫分析法测定了体外灌注的人脐血管流出物中四种主要前列腺素（6-酮-前列腺素F1α、前列腺素E2、前列腺素F2α和血栓素B2）的含量。通过扫描电子显微镜（SEM）评估，仅发现少量血小板附着于血管壁。在用浓度逐渐降低的所有四种前列腺素进行初始冲洗后，进入了一个稳定阶段，持续4至5小时。在此阶段，吲哚美辛可抑制其生成，而花生四烯酸仅能轻微刺激其生成。血栓素A2合成酶抑制剂UK 38485可降低血栓素B2的生成，而在非常高的浓度下仅对其他三种前列腺素有轻微影响。动脉产生的6-酮-前列腺素F1α相对比静脉更多。
