Thanuja B, Parimalavalli R, Vijayanand S, Alharbi Reem Mohammed, Abdel-Raouf Neveen, Ibraheem Ibraheem Borie M, Sholkamy Essam Nageh, Durairaj Kaliannan, Meansbo Hadish Kibrom
Department of Food Science and Nutrition, Periyar University, Salem 636 011, Tamil Nadu, India.
Department of Biotechnology, Thiruvalluvar University, Serkadu, Vellore, India.
Evid Based Complement Alternat Med. 2022 Feb 21;2022:8575026. doi: 10.1155/2022/8575026. eCollection 2022.
This study is intended to evaluate the cytotoxicity of native and dual-modified black rice flour against the colon cancer cell line (HCT116) and mouse embryo cell line (3T3-L1) by using the MTT assay. The modification techniques applied to prepare rice flour samples were enzymatic modification and heat moisture treatment. In this study, the IC50 of native black rice flour and modified black rice flour was 255.78 g/mL and 340.85 g/mL, respectively. The result confirms that the native black rice flour has significant cytotoxic and anticancer potential against human colon cancer cells. In addition, the IC50 of native black rice flour and modified black rice flour on the 3T3-L1 cell line was found to be 345.96 g/mL and 1106.94 g/mL, respectively. The results showed that the native black rice flour had weak cytotoxicity, and modified black rice flour was nontoxic in both the cell lines. The active component of phytochemicals present in black rice flour has a potential role in preventing colon cancer.
本研究旨在通过MTT法评估天然黑米面粉和双重改性黑米面粉对结肠癌细胞系（HCT116）和小鼠胚胎细胞系（3T3-L1）的细胞毒性。用于制备米粉样品的改性技术为酶法改性和湿热处理。在本研究中，天然黑米面粉和改性黑米面粉的IC50分别为255.78 g/mL和340.85 g/mL。结果证实，天然黑米面粉对人结肠癌细胞具有显著的细胞毒性和抗癌潜力。此外，发现天然黑米面粉和改性黑米面粉对3T3-L1细胞系的IC50分别为345.96 g/mL和1106.94 g/mL。结果表明，天然黑米面粉具有较弱的细胞毒性，而改性黑米面粉在两种细胞系中均无毒。黑米面粉中存在的植物化学活性成分在预防结肠癌方面具有潜在作用。
