Department of Pharmacy, The People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi, China.
Institute of Green Algae, Longhua Branch, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, 518120 Guangdong, China.
Comput Math Methods Med. 2022 Feb 21;2022:9604456. doi: 10.1155/2022/9604456. eCollection 2022.
To investigate the potential pharmacological value of extracts from honeysuckle on patients with mild coronavirus disease 2019 (COVID-19) infection.
The active components and targets of honeysuckle were screened by Traditional Chinese Medicine Database and Analysis Platform (TCMSP). SwissADME and pkCSM databases predict pharmacokinetics of ingredients. The Gene Expression Omnibus (GEO) database collected transcriptome data for mild COVID-19. Data quality control, differentially expressed gene (DEG) identification, enrichment analysis, and correlation analysis were implemented by R toolkit. CIBERSORT evaluated the infiltration of 22 immune cells.
The seven active ingredients of honeysuckle had good oral absorption and medicinal properties. Both the active ingredient targets of honeysuckle and differentially expressed genes of mild COVID-19 were significantly enriched in immune signaling pathways. There were five overlapping immunosignature genes, among which RELA and MAP3K7 expressions were statistically significant ( < 0.05). Finally, immune cell infiltration and correlation analysis showed that RELA, MAP3K7, and natural killer (NK) cell are with highly positive correlation and highly negatively correlated with hematopoietic stem cells.
Our analysis suggested that honeysuckle extract had a safe and effective protective effect against mild COVID-19 by regulating a complex molecular network. The main mechanism was related to the proportion of infiltration between NK cells and hematopoietic stem cells.
研究金银花提取物对轻度 2019 年冠状病毒病（COVID-19）感染患者的潜在药理价值。
通过中药数据库与分析平台（TCMSP）筛选金银花的活性成分和靶点。SwissADME 和 pkCSM 数据库预测成分的药代动力学。基因表达综合数据库（GEO）收集轻度 COVID-19 的转录组数据。通过 R 工具包进行数据质量控制、差异表达基因（DEG）鉴定、富集分析和相关性分析。CIBERSORT 评估 22 种免疫细胞的浸润。
金银花的七种活性成分具有良好的口服吸收性和药效。金银花的活性成分靶点和轻度 COVID-19 的差异表达基因均显著富集于免疫信号通路。有五个重叠的免疫特征基因，其中 RELA 和 MAP3K7 的表达具有统计学意义（<0.05）。最后，免疫细胞浸润和相关性分析表明，RELA、MAP3K7 和自然杀伤（NK）细胞与造血干细胞之间具有高度正相关和高度负相关。
我们的分析表明，金银花提取物通过调节复杂的分子网络对轻度 COVID-19 具有安全有效的保护作用。主要机制与 NK 细胞和造血干细胞之间的浸润比例有关。
