用于检测血管炎症和动脉粥样硬化中内皮细胞活化的单核细胞黏附测定。
Monocyte Adhesion Assays for Detecting Endothelial Cell Activation in Vascular Inflammation and Atherosclerosis.
机构信息
Cardiovascular Research Center, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA.
Northeastern University, Boston, MA, USA.
出版信息
Methods Mol Biol. 2022;2419:169-182. doi: 10.1007/978-1-0716-1924-7_10.
Abstract
Monocyte adhesion assay, a fluorescence-based method, enables the detection and quantification of monocyte adhesion to endothelial cell (EC) monolayers in vitro and measures EC activation. We describe in this chapter a monocyte adhesion assay based on two published papers from our laboratory that can be effectively used in studying the mechanisms of both pro- and anti-inflammatory cytokines in EC activation. Endothelial cell monolayers are cultured and treated with desired drug, cytokines, or other stimuli and incubated with fluorescently labeled monocytes.
摘要
单核细胞黏附实验是一种基于荧光的方法，可用于检测和定量体外单核细胞黏附在内皮细胞（EC）单层上的情况，并可测量 EC 的激活程度。本章介绍了一种基于我们实验室的两篇已发表论文的单核细胞黏附实验方法，该方法可有效地用于研究促炎和抗炎细胞因子在 EC 激活中的作用机制。将内皮细胞单层进行培养并使用所需的药物、细胞因子或其他刺激物进行处理，然后与荧光标记的单核细胞孵育。
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