a Laboratory for Atherosclerosis Research, Institute for Clinical and Experimental Medicine , Prague , Czech Republic.
b Department of Physiology, Faculty of Science, Charles University , Prague , Czech Republic.
Cell Adh Migr. 2019 Dec;13(1):293-302. doi: 10.1080/19336918.2019.1644856.
Visceral adipose tissue (VAT) may play a critical role in atherosclerotic cardiovascular disease. The goal of this study was to determine the effect of human VAT-released pro‑inflammatory cytokines on monocyte adhesion to the endothelium. The cytokine effects on monocyte adhesion to the endothelial cells (ECs) were tested using adipose tissue-conditioned media (ATCM) prepared by culturing human VAT. The cytokines concentrations in ATCM, the cytokines expression and adhesion molecules in stimulated ECs were measured. The concentrations of IL-1β,TNF-α,MCP-1,IL-10,and RANTES measured in ATCM correlated positively with monocyte adhesiveness to ECs. Additionally, ATCM increased the adhesion molecules (ICAM-1, VCAM-1) gene expression. Selective inhibitors highlighted the importance of IL-1β and TNF-α in the process by a significant decrease in monocyte adhesion compared to ATCM preconditioning without inhibitors. Human VAT significantly increased monocyte adhesion to ECs. It was significantly influenced by IL-1β, TNF-α, MCP-1, IL-10, and RANTES, with IL-1β and TNF‑α having the strongest impact.
内脏脂肪组织（VAT）可能在动脉粥样硬化性心血管疾病中起关键作用。本研究旨在确定人 VAT 释放的促炎细胞因子对单核细胞黏附内皮的影响。使用培养人 VAT 制备的脂肪组织条件培养基（ATCM）测试细胞因子对单核细胞黏附内皮细胞（EC）的影响。测量 ATCM 中的细胞因子浓度、刺激 EC 中的细胞因子表达和黏附分子。ATCM 中测量的 IL-1β、TNF-α、MCP-1、IL-10 和 RANTES 浓度与单核细胞对 EC 的黏附性呈正相关。此外，ATCM 增加了黏附分子（ICAM-1、VCAM-1）的基因表达。选择性抑制剂强调了 IL-1β 和 TNF-α 在该过程中的重要性，与没有抑制剂的 ATCM 预处理相比，单核细胞黏附显著减少。人 VAT 显著增加单核细胞对 EC 的黏附。它受到 IL-1β、TNF-α、MCP-1、IL-10 和 RANTES 的显著影响，其中 IL-1β 和 TNF-α 的影响最大。
