Krumbacher K, van der Feltz M J, Happel M, Gerlach C, Lösslein L K, Grosse-Wilde H
Tissue Antigens. 1986 May;27(5):262-8. doi: 10.1111/j.1399-0039.1986.tb01531.x.
Peripheral blood mononuclear cells from DLA typed dogs were treated with rabbit-anti-dog-beta 2-microglobulin and subsequently with goat-anti-rabbit-immunoglobulin in order to aggregate the DLA class I molecules on the cell membrane (lysostrip). Utilizing a panel of 70 defined DLA-A and DLA-B antisera, lymphocytes treated in this way showed resistance to complement dependent lysis with monospecific DLA-A sera only, whereas reactivity of DLA-B antisera was not blocked; on the contrary, complete lympholysis with each DLA-B antiserum was recognized. Thus, the DLA-B antigens, evidently not associated with beta 2-microglobulin, are designated as candidates for class II gene products. The different reactions of DLA-C antisera after lysostrip did not allow a precise assignment of this antigen series as yet.
对经犬白细胞抗原（DLA）分型的犬外周血单个核细胞先用兔抗犬β2-微球蛋白处理，随后用山羊抗兔免疫球蛋白处理，以聚集细胞膜上的DLA I类分子（溶菌剥离）。使用一组70种已知的DLA - A和DLA - B抗血清，经这种方式处理的淋巴细胞仅对单特异性DLA - A血清介导的补体依赖性细胞溶解有抗性，而DLA - B抗血清的反应性未被阻断；相反，每种DLA - B抗血清均能引起完全的淋巴细胞溶解。因此，显然不与β2-微球蛋白相关的DLA - B抗原被指定为II类基因产物的候选者。溶菌剥离后DLA - C抗血清的不同反应目前还无法对该抗原系列进行精确归类。
