Wagner J L, Works J D, Storb R
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.
Tissue Antigens. 1998 Oct;52(4):397-401. doi: 10.1111/j.1399-0039.1998.tb03063.x.
The dog has been an important model for solid organ and hematopoietic stem cell transplantation for over 30 years. Fundamental to the continuing usage of the model is the development of molecular-based histocompatibility typing of donors and recipients. Previous histocompatibility typing methods used in the dog have not been precise enough to identify dog leukocyte antigen (DLA)-matched unrelated dogs. This study was undertaken to begin the process of identifying DLA-matched unrelated dogs. In this study polymerase chain reaction-single-stranded conformational polymorphism is used to separate alleles thereby allowing sequenced-based typing of the two most polymorphic class II genes described to date in the dog DLA-DRB1 and DLA-DQB1.
30多年来，狗一直是实体器官和造血干细胞移植的重要模型。该模型持续应用的基础是供体和受体基于分子的组织相容性分型技术的发展。以往用于狗的组织相容性分型方法不够精确，无法识别狗白细胞抗原（DLA）匹配的无关狗。本研究旨在开始识别DLA匹配的无关狗的进程。在本研究中，聚合酶链反应-单链构象多态性用于分离等位基因，从而对狗DLA-DRB1和DLA-DQB1中迄今描述的两个多态性最高的II类基因进行基于测序的分型。
