Bruhl J J, Ashford A E
Stain Technol. 1986 May;61(3):163-7. doi: 10.3109/10520298609110727.
Aldehyde fixation and glycol methacrylate embedding were applied to herbarium specimens of fruits of the Compositae. Sections 1-2 micron thick were cut with glass knives. Softening was unnecessary and the hydrophilic properties of the resin permitted staining with a number of dyes. Specimens were examined with bright field and polarized light microscopy. The technique gives good structural preservation and resolution even with 81-year-old herbarium material.
对菊科果实的标本馆标本采用醛固定和乙二醇甲基丙烯酸酯包埋法。用玻璃刀切成1-2微米厚的切片。无需软化处理,树脂的亲水性允许用多种染料染色。用明场和偏光显微镜检查标本。即使对于有81年历史的标本馆材料,该技术也能实现良好的结构保存和分辨率。