Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Siming South Road 422, Xiamen 361005, China.
Anal Chem. 2022 Mar 15;94(10):4201-4208. doi: 10.1021/acs.analchem.1c04210. Epub 2022 Mar 3.
Proton nuclear magnetic resonance (H NMR) spectroscopy presents a powerful detection tool for studying chemical compositions and molecular structures. In practical chemical and biological applications, H NMR experiments are generally confronted with the challenge of spectral congestions caused by abundant observable components and intrinsic limitations of a narrow frequency distribution range and extensive coupling splitting. Herein, a one-dimensional (1D) general NMR method is proposed to individually extract the signals of targeted proton groups based on their endogenous spin singlet states excited from coupling interactions, and it is suitable for high-resolution detections on complex chemical and biological samples. The applicability of the proposed method is demonstrated by experimental observations on chemical solutions containing different coupled components, intact grape tissues subjected to crowded resonances, and pig brain with various metabolites. Moreover, the proposed method is further exploited for magnetic resonance spectroscopy applications by directly combining the spatial localization module, showing promise in biological metabolite studies.
质子核磁共振（H NMR）光谱学为研究化学组成和分子结构提供了一种强大的检测工具。在实际的化学和生物学应用中，H NMR 实验通常面临着由于丰富的可观测成分以及窄频率分布范围和广泛的耦合分裂的内在限制而导致的光谱拥挤的挑战。本文提出了一种一维（1D）通用 NMR 方法，该方法基于来自耦合相互作用的内源性自旋单态，可单独提取目标质子基团的信号，适用于复杂化学和生物样品的高分辨率检测。通过对含有不同耦合成分的化学溶液、受到拥挤共振的完整葡萄组织和具有各种代谢物的猪脑进行实验观察，验证了该方法的适用性。此外，该方法通过直接结合空间定位模块，进一步应用于磁共振波谱学应用，在生物代谢物研究中具有广阔的应用前景。
