黑米花青素纳米复合材料的合成、表征及其对甲氨蝶呤诱导大鼠肝毒性的保护作用。
Synthesis, characterization and protection effect of black rice anthocyanins nano-composite against hepatotoxicity induced by methotrexate in rats.
机构信息
Imam Abdulrahman Bin Faisal University, College of Science, Department of Chemistry, Dammam, Saudi Arabia.
Agricultural Research Center, Food Technology Research Institute, Crops Technology Research Department, Giza, Egypt.
出版信息
Braz J Biol. 2022 Mar 7;84:e248726. doi: 10.1590/1519-6984.248726. eCollection 2022.
The present study aimed to investigate the beneficial of prepared black rice anthocyanins nano-composite (An-AgNps) against hepatotoxicity induced by methotrexate (MTX) in rats. Anthocyanins nano-composite was prepared by silver as the metallic ion reduction and were characterized by IR and SEM. The rats in our experiment were divided into five groups. Serum lipid profile, serum transaminases (ALT and AST), ALP, LDH, TBA, GSH and SOD were examined. The results show that SEM of An-AgNps has average particle size from 70 to 130nm. In the group treated with MTX; TC, TG, LDL-c, ALT, AST, ALP, LDH and TBA levels were significantly (P≤0.05) increased than NC, while, HDL-c, SOD and GSH levels were significantly (P≤0.05) decreased. On the other hand, An-AgNps + MTX treated groups were reversed the levels of all biomarkers similar to NC. In conclusion, the results show that An-AgNps has a protective effect on MTX-induced hepatotoxicity and oxidative stress.
本研究旨在探讨黑米花色苷纳米复合物（An-AgNps）对甲氨蝶呤（MTX）诱导的大鼠肝毒性的有益作用。花色苷纳米复合物通过银作为金属离子还原制备，并通过 IR 和 SEM 进行了表征。实验中的大鼠分为五组。检测了血清脂质谱、血清转氨酶（ALT 和 AST）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）、总胆汁酸（TBA）、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）。结果表明，An-AgNps 的 SEM 具有平均粒径为 70 至 130nm。在接受 MTX 治疗的组中；TC、TG、LDL-c、ALT、AST、ALP、LDH 和 TBA 水平明显（P≤0.05）高于 NC，而 HDL-c、SOD 和 GSH 水平明显（P≤0.05）降低。另一方面，An-AgNps+MTX 治疗组使所有生物标志物的水平恢复到类似 NC 的水平。总之，结果表明 An-AgNps 对 MTX 诱导的肝毒性和氧化应激具有保护作用。
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