Alghad College for Applied Medical Sciences, Dammam, Saudi Arabia.
Agricultural Research Center, Food Technology Research Institute, Crops Technology Research Department, Giza, Egypt.
Braz J Biol. 2024 Aug 5;84:e280570. doi: 10.1590/1519-6984.280570. eCollection 2024.
Anthocyanins are known as an antioxidant, and their water-soluble purple-colored pigments are very nutritive. Therefore, the present study investigated the antioxidant activity of black rice anthocyanins nano-composite against infertility induced by AlCl3 in rats. Anthocyanin silver nanoparticles (An-AgNPs) were prepared by reducing black rice anthocyanin with the metallic ions. Antioxidant activity (DPPH %) of anthocyanin was determined. Also, the morphology of (An-AgNPs) was examined by scanning electron microscopy (SEM). Albino rats were divided into five groups (negative control (NC): fed on basel diet, positive control (PC): treated with AlCl3 (34 mg/kg bw) for seventy days, and three other groups treated with AlCl3 (34 mg/kg bw) + An-AgNPs at 10, 15, and 20 mg/kg, b.w/ day, respectively for seventy days. Serum testosterone, LH, FSH, and estradiol were measured. Additionally, Sperm motility, Sperm count (Testicular and Epididymal), fructose in semen, and semen quality were determined. The values of the anthocyanin component and DPPH radical scavenging activity obtained were 3603.82±6.11 mg CCE/g and 84.62±1.98, respectively. An-AgNPs shows tend to agglomerate, particles are uniform in size and shape, and the diameter of the particles ranges between 70nm to 130nm. LH, estradiol and testosterone levels increased significantly in rats treated with An-AgNPs 10, 15, 20 mg/kg b.w+ AlCl3 (34 mg/kg bw) also exhibited significantly higher sperm motility, sperm count, and daily sperm production, and decreased sperm transit rate than G2. In comparison to G2, animals treated with AlCl3 (34 mg/kg bw) + An-AgNPs 10, 15, 20 mg/kg b.w(G3 to G5) had significantly higher semen and semen quality (P 0.05). We can conclude that the An-AgNPs showed a strong effect against infertility induced by AlCl3; this represents a suitable natural supply of biological substances for medicine and anthocyanins could be considered the ideal ingredients against oxidative stress-induced infertility.
花色苷是一种抗氧化剂，其水溶性紫色色素非常有营养。因此，本研究调查了黑米花色苷纳米复合物对氯化铝诱导的大鼠不育的抗氧化活性。花色苷银纳米粒子（An-AgNPs）是通过用金属离子还原黑米花色苷制备的。测定花色苷的抗氧化活性（DPPH%）。此外，通过扫描电子显微镜（SEM）检查（An-AgNPs）的形态。白化大鼠分为五组（阴性对照组（NC）：用基础饲料喂养，阳性对照组（PC）：用 AlCl3（34mg/kg bw）处理 70 天，另外三组用 AlCl3（34mg/kg bw）+An-AgNPs 分别以 10、15 和 20mg/kg bw/天处理 70 天。测量血清睾酮、LH、FSH 和雌二醇。此外，还测定了精子活力、睾丸和附睾精子计数、精液中的果糖和精液质量。获得的花色苷成分和 DPPH 自由基清除活性的值分别为 3603.82±6.11mg CCE/g 和 84.62±1.98。An-AgNPs 趋于聚集，颗粒大小均匀，形状一致，颗粒直径在 70nm 到 130nm 之间。用 An-AgNPs 10、15、20mg/kg bw+AlCl3（34mg/kg bw）处理的大鼠的 LH、雌二醇和睾酮水平显著升高，精子活力、精子计数和每日精子产量也显著升高，精子转运率降低与 G2 相比，用 AlCl3（34mg/kg bw）+An-AgNPs 10、15、20mg/kg bw 处理的动物（G3 至 G5）的精液和精液质量显著提高（P<0.05）。我们可以得出结论，An-AgNPs 对 AlCl3 诱导的不育有很强的作用；这代表了一种合适的天然生物物质供应，可用于医学，花色苷可以被认为是对抗氧化应激诱导的不育的理想成分。
