嘌呤核苷对腹水细胞核酸合成的影响。

Effects of purine riboside on nucleic acid synthesis in ascites cells.

作者信息

Bohr V

出版信息

Biochim Biophys Acta. 1978 Jun 22;519(1):125-37. doi: 10.1016/0005-2787(78)90067-9.

Abstract

Purine riboside (nebularine, 9-beta-ribofuranosylpurine) is a naturally occurring base analog which closely resembles adenosine. It inhibits carcinogenic growth. Purine riboside strongly inhibits RNA and DNA synthesis in different cancer ascites cells. Gel electrophoretic analysis of RNA synthesis in vivo in the presence of purine riboside shows the ribosomal components to be inhibited the most. A method for assaying purine riboside or its phosphates intracellularly has been devised, and by using this it has been shown that purine riboside is extensively phosphorylated in the cells. The triphosphate derivative of purine riboside has been isolated and tested in the Escherichia coli RNA polymerase assay. It appears not to be incorporated into this type of RNA and to competitively inhibit this reaction with regard to ATP.

摘要

嘌呤核苷（喷司他丁，9-β-D-呋喃核糖基嘌呤）是一种天然存在的碱基类似物，与腺苷非常相似。它能抑制致癌生长。嘌呤核苷强烈抑制不同癌细胞腹水细胞中的RNA和DNA合成。在嘌呤核苷存在下对体内RNA合成进行的凝胶电泳分析表明，核糖体成分受到的抑制最为明显。已经设计出一种用于细胞内测定嘌呤核苷或其磷酸盐的方法，通过使用该方法已表明嘌呤核苷在细胞中被广泛磷酸化。嘌呤核苷的三磷酸衍生物已被分离出来，并在大肠杆菌RNA聚合酶测定中进行了测试。它似乎不会掺入这种类型的RNA中，并且在ATP方面竞争性抑制该反应。