Chobit'ko V G, Zakharova N B, Rubin V I
Vopr Med Khim. 1986 May-Jun;32(3):118-21.
Erythrocytes, obtained from blood of 12 patients with diabetes mellitus and of 6 donors, were incubated in isotonic medium at 37 degrees within 180 min. Patterns of thiamin metabolism (activity of transketolase and TDP-effect), of glycolysis (content of glucose, lactate) and of energy metabolism (ATP, ADP, AMP and 2,3-DPG) were studied during the incubation. The ratios of energy-synthesizing and energy-consuming reactions were shifted towards the latter reactions in erythrocytes of the patients, which appears to be among the factors responsible for impairment of thiamin metabolism in diabetes. In order to correct the impairments found some drugs, such as insulin, thiamin, cocarboxylase, thiamin combined with adenine were added into the incubation medium. Insulin and cocarboxylase as well as insulin combined with thiamin and adenine exhibited the best effect on the patterns studied; these drugs normalized the vitamin B1 metabolism and improved the parameters of energy metabolism within 120 min of incubation in erythrocytes isolated from blood of patients with diabetes mellitus.
从12名糖尿病患者和6名献血者的血液中获取红细胞，将其在37摄氏度的等渗培养基中孵育180分钟。在孵育过程中研究了硫胺素代谢模式（转酮醇酶活性和TDP效应）、糖酵解（葡萄糖、乳酸含量）和能量代谢（ATP、ADP、AMP和2,3 - DPG）。患者红细胞中能量合成反应与能量消耗反应的比例向后者偏移，这似乎是糖尿病中硫胺素代谢受损的因素之一。为了纠正所发现的损伤，将一些药物，如胰岛素、硫胺素、羧化辅酶、硫胺素与腺嘌呤的组合添加到孵育培养基中。胰岛素和羧化辅酶以及胰岛素与硫胺素和腺嘌呤的组合对所研究的模式表现出最佳效果；这些药物在从糖尿病患者血液中分离出的红细胞孵育120分钟内使维生素B1代谢正常化并改善了能量代谢参数。
