用亚临界水获得的枸杞多糖-蛋白质缀合物稳定的硒纳米颗粒的性质
Properties of selenium nanoparticles stabilized by Lycium barbarum polysaccharide-protein conjugates obtained with subcritical water.
作者信息
Zhang Jixian, Ji Tao, Yang Xue, Liu Guoyan, Liang Li, Liu Xiaofang, Wen Chaoting, Ye Zhiqiang, Wu Maowei, Xu Xin
机构信息
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.
出版信息
Int J Biol Macromol. 2022 Apr 30;205:672-681. doi: 10.1016/j.ijbiomac.2022.02.165. Epub 2022 Feb 28.
Selenium nanoparticles (SeNPs) in an aqueous solution have poor stability and tend to aggregate when stored for a long time. In the present study, SeNPs were stabilized by using Lycium barbarum polysaccharide (LBP) and Lycium barbarum protein (LBPr) conjugates (LBPP) as a stabilizer and dispersing agent. Particularly, the LBPP was obtained with subcritical water treatment. In addition, the physical stability, re-dispersity and antitumor activity of LBPP-SeNPs were investigated. The results showed the particle size of LBPP-SeNPs was maintained at 111.5-117 nm, which was stable at PH 6, 4 °C and darkness for at least 40 days. Besides, the result of TEM showed that the dispersion of LBPP-SeNPs had more clear layers and smoother surfaces. Moreover, LBPP-SeNPs had excellent re-dispersibility and exhibited a significant inhibitory effect on HepG-2 cells and Caco-2 cells, respectively (p < 0.05). Therefore, LBPP-SeNPs can be used as potential selenium nutritional supplements for food and medical applications.
水溶液中的硒纳米颗粒（SeNPs）稳定性较差，长时间储存时容易聚集。在本研究中，通过使用枸杞多糖（LBP）和枸杞蛋白（LBPr）共轭物（LBPP）作为稳定剂和分散剂来稳定SeNPs。特别地，LBPP是通过亚临界水处理获得的。此外，还研究了LBPP-SeNPs的物理稳定性、再分散性和抗肿瘤活性。结果表明，LBPP-SeNPs的粒径保持在111.5-117nm，在pH 6、4℃和黑暗条件下至少40天稳定。此外，透射电镜结果表明，LBPP-SeNPs的分散层更清晰，表面更光滑。而且，LBPP-SeNPs具有优异的再分散性，分别对HepG-2细胞和Caco-2细胞表现出显著的抑制作用（p<0.05）。因此，LBPP-SeNPs可作为潜在的硒营养补充剂用于食品和医疗应用。
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