Clinical Laboratory Center, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
Geriatrics Department, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
Math Biosci Eng. 2022 Jan 18;19(3):3022-3035. doi: 10.3934/mbe.2022139.
Single-stranded DNA-binding protein 1 (SSBP1) plays an important role in DNA repair processes and the maintenance of genomic stability. The aim of this study was to evaluate the expression of SSBP1 and its prognostic value in lung adenocarcinoma (LUAD) using bioinformatics approaches.
We applied databases including UALCAN, Kaplan-Meier plotter, LinkedOmics, Webgestalt, cBioPortal and TIMER2.0 in this study.
We found that SSBP1 expression was up-regulated in LUAD samples and was correlated with clinicopathological features including age, cancer stage, and nodal metastasis status by the UALCAN analysis. Multivariate Cox regression analysis by the Kaplan-Meier plotter showed that high SSBP1 expression was independently correlated with poor overall survival (hazard ratio = 1.63, 95% confidence interval: 1.08-2.46, logrank P = 0.02). The LinkedOmics analysis showed that 5078 genes were positively correlated with SSBP1 expression, whereas 7905 genes were negatively correlated with SSBP1 in LUAD. Functional enrichment analysis using the Webgestalt tool showed that for SSBP1 and the genes positively correlating with it, the significantly enriched biological process was ribosomal large subunit biogenesis, and the significantly enriched pathway was proteasome. According to the cBioPortal database, the frequency of SSBP1 alterations was 1.7% in LUAD patients, and patients with SSBP1 alterations had worse prognosis (logrank P = 4.26e-05) compared with those unaltered for SSBP1. Finally, SSBP1 expression was negatively correlated with B cell infiltration level (Rho = -0.193, P = 1.54e-05) and the expression of B cell biomarkers including CD79A and CD19.
Our results suggest that SSBP1 may be a prognostic marker for human LUAD.
单链 DNA 结合蛋白 1（SSBP1）在 DNA 修复过程和基因组稳定性的维持中发挥着重要作用。本研究旨在通过生物信息学方法评估 SSBP1 在肺腺癌（LUAD）中的表达及其预后价值。
我们在这项研究中应用了包括 UALCAN、Kaplan-Meier plotter、LinkedOmics、Webgestalt、cBioPortal 和 TIMER2.0 在内的数据库。
我们发现 SSBP1 在 LUAD 样本中表达上调，UALCAN 分析表明其与年龄、癌症分期和淋巴结转移状态等临床病理特征相关。Kaplan-Meier plotter 的多变量 Cox 回归分析显示，高 SSBP1 表达与总生存期不良独立相关（危险比=1.63，95%置信区间：1.08-2.46，对数秩检验 P=0.02）。LinkedOmics 分析表明，在 LUAD 中，5078 个基因与 SSBP1 表达呈正相关，而 7905 个基因与 SSBP1 表达呈负相关。Webgestalt 工具的功能富集分析显示，对于 SSBP1 和与其呈正相关的基因，显著富集的生物学过程是核糖体大亚基生物发生，显著富集的途径是蛋白酶体。根据 cBioPortal 数据库，LUAD 患者中 SSBP1 改变的频率为 1.7%，与 SSBP1 未改变的患者相比，SSBP1 改变的患者预后更差（对数秩检验 P=4.26e-05）。最后，SSBP1 表达与 B 细胞浸润水平呈负相关（Rho=-0.193，P=1.54e-05），与 B 细胞标志物如 CD79A 和 CD19 的表达呈负相关。
我们的研究结果表明，SSBP1 可能是 LUAD 患者的一个预后标志物。
