Tan Zhibo, Chen Min, Wang Ying, Peng Feng, Zhu Xiaopeng, Li Xin, Zhang Lei, Li Ying, Liu Yajie
Department of Radiation Oncology, Peking University Shenzhen Hospital, no. 1120, Lianhua Road, Futian District, Shenzhen, Guangdong Province, 518036, China.
Shenzhen Key Laboratory of Gastrointestinal Cancer Translational Research, Cancer Institute, Shenzhen-Peking University-Hong Kong University of Science & Technology Medical Center, Peking University Shenzhen Hospital, No. 1120, Lianhua Road, Futian District, Shenzhen, Guangdong Province, 518036, China.
Biomark Med. 2022 Feb;16(2):83-100. doi: 10.2217/bmm-2021-0919. Epub 2021 Dec 9.
The study aims to pinpoint hub genes and investigate their functions in order to gain insightful understandings of lung adenocarcinoma (LUAD). Bioinformatic approaches were adopted to investigate genes in databases including Gene Expression Omnibus, WebGestalt, STRING and Cytoscape, GEPIA2, Oncomine, Human Protein Atlas, TIMER2.0, UALCAN, cBioPortal, TargetScanHuman, OncomiR, ENCORI, Kaplan-Meier plotter, UCSC Xena, European Molecular Biology Laboratory - European Bioinformatics Institute Single Cell Expression Atlas and CancerSEA. Five hub genes were ascertained. was overexpressed in a range of cancers, including LUAD. Promoter methylation, amplification and miRNA regulation might trigger upregulation, signaling poor prognosis. with its coexpressed genes were enriched in the cell cycle pathway. Intratumor heterogeneity of expression could be observed. Cell clusters with expression were more prone to metastasis and epithelial-to-mesenchymal transition. might potentially act as a prognostic biomarker for LUAD.
该研究旨在确定关键基因并研究其功能，以便深入了解肺腺癌（LUAD）。采用生物信息学方法在包括基因表达综合数据库、WebGestalt、STRING和Cytoscape、GEPIA2、Oncomine、人类蛋白质图谱、TIMER2.0、UALCAN、cBioPortal、TargetScanHuman、OncomiR、ENCORI、Kaplan-Meier绘图仪、UCSC Xena、欧洲分子生物学实验室-欧洲生物信息学研究所单细胞表达图谱和CancerSEA等数据库中研究基因。确定了五个关键基因。在包括肺腺癌在内的一系列癌症中过表达。启动子甲基化、扩增和miRNA调控可能引发上调，预示预后不良。及其共表达基因在细胞周期途径中富集。可观察到表达的肿瘤内异质性。具有表达的细胞簇更容易发生转移和上皮-间质转化。可能潜在地作为肺腺癌的预后生物标志物。
