Department of Gastroenterology, Shijiazhuang People's Hospital, Shijiazhuang City, P. R. China.
Bioengineered. 2022 Mar;13(3):6781-6793. doi: 10.1080/21655979.2022.2034565.
Although long non-coding RNAs (lncRNAs) have been demonstrated to be dysregulated in gastric cancer (GC), the function of lncRNA HCG18 (HCG18) in GC is elusive. Therefore, the study was designed to evaluate the underlying mechanism of HCG18 in GC. HCG18 and microRNA 146a-5p (miR-146a-5p) levels in GC were evaluated by RT-qPCR. The effects of miR-146a-5p and HCG18 on GC cell function were examined using Transwell assay, colony formation, and CCK-8 assays. Tumor necrosis factor receptor-associated factor 6 (TRAF6) and p65 expression levels were detected by Western blot. HCG18 and miR-146a-5p target genes were identified using luciferase reporter and bioinformatics assays. HCG18 expression was increased in GC. HCG18 overexpression significantly increased GC cell proliferation, invasion, and migration. Furthermore, HCG18 overexpression inhibited miR-146a-5p and upregulated TRAF6 and p65 expression. Finally, miR-146a-5p/TRAF6 was found to be involved in the role of HCG18 in GC progression . Altogether, HCG18 promotes GC progression via the miR-146a-5p/TRAF6 axis and could be a GC treatment target.
虽然长链非编码 RNA（lncRNA）在胃癌（GC）中已被证明失调，但 lncRNA HCG18（HCG18）在 GC 中的功能尚不清楚。因此，本研究旨在评估 HCG18 在 GC 中的潜在机制。通过 RT-qPCR 评估 GC 中 HCG18 和 microRNA 146a-5p（miR-146a-5p）的水平。使用 Transwell 测定、集落形成和 CCK-8 测定检测 miR-146a-5p 和 HCG18 对 GC 细胞功能的影响。通过 Western blot 检测肿瘤坏死因子受体相关因子 6（TRAF6）和 p65 表达水平。使用荧光素酶报告和生物信息学测定鉴定 HCG18 和 miR-146a-5p 的靶基因。HCG18 在 GC 中表达增加。HCG18 过表达显著增加 GC 细胞的增殖、侵袭和迁移。此外，HCG18 过表达抑制 miR-146a-5p 并上调 TRAF6 和 p65 表达。最后，发现 miR-146a-5p/TRAF6 参与了 HCG18 在 GC 进展中的作用。总之，HCG18 通过 miR-146a-5p/TRAF6 轴促进 GC 进展，可能成为 GC 的治疗靶点。
