Department of Nursing, Chung-Jen Junior College of Nursing, Health Sciences and Management, Chiayi, Taiwan, R.O.C.
School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C.
In Vivo. 2022 Mar-Apr;36(2):713-722. doi: 10.21873/invivo.12757.
BACKGROUND/AIM: Natural skin whiteners have been investigated for centuries. The development of preparations that safely achieve whitening of hyper-pigmented skin lesions is a challenge for the cosmetics industry. Furthermore, promoting rapid wound healing and minimizing inflammation in injured skin are key to prevent from abnormal pigmentation in scar tissue. Natural products, including the fungus Tremella fuciformis (TF), are attracting attention as potential sources of lead compounds for these applications.
We investigated the in vitro effects of TF on melanogenesis in murine B16F10 cells. Melanin and tyrosinase levels were measured after treatment with TF. Wound healing in human keratinocytes (HaCaT) and fibroblasts (Detroit 551) was also determined via cell migration assay prior to TF exposure.
TF significantly decreased melanin content and tyrosinase expression in a concentration-dependent manner in B16F10 cells. Furthermore, TF promoted wound healing in human HaCaT keratinocytes and Detroit 551 fibroblasts.
TF proved effectively on inhibiting melanogenesis and promoting wound healing in vitro, demonstrating its potential as a novel skin-whitening agent. However, further clinical studies of safety and efficacy are required.
背景/目的：天然皮肤增白剂已经研究了几个世纪。开发能够安全实现皮肤色素沉着过度病变增白的制剂是化妆品行业面临的挑战。此外，促进伤口快速愈合和减少受伤皮肤的炎症是防止疤痕组织中异常色素沉着的关键。天然产品，包括真菌银耳（TF），作为这些应用的潜在先导化合物来源引起了人们的关注。
我们研究了 TF 在体外对小鼠 B16F10 细胞黑素生成的影响。用 TF 处理后测量黑色素和酪氨酸酶水平。在 TF 暴露之前，还通过细胞迁移测定法测定了人角质形成细胞（HaCaT）和成纤维细胞（Detroit 551）中的伤口愈合情况。
TF 显著地以浓度依赖性方式降低 B16F10 细胞中的黑色素含量和酪氨酸酶表达。此外，TF 促进了人 HaCaT 角质形成细胞和 Detroit 551 成纤维细胞的伤口愈合。
TF 在体外有效抑制黑素生成和促进伤口愈合，表明其作为新型皮肤增白剂的潜力。然而，需要进一步进行安全性和疗效的临床研究。
