Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, Japan.
Molecules. 2021 Oct 1;26(19):5959. doi: 10.3390/molecules26195959.
Melanin is a natural pigment produced by cells to prevent damage caused by ultraviolet radiation. Previously, resveratrol was shown to reduce melanin synthesis. As a natural polyphenol with various biological activities, resveratrol occurs in a variety of beverages and plant foods, such as grapes. Therefore, we investigated whether grape extracts containing resveratrol also had the ability to regulate melanin synthesis. In this study, we used mouse B16F10 melanoma cells as a model for melanin synthesis with the melanogenesis-inducing α-melanocyte-stimulating hormone (α-MSH) as a positive control. Our results confirmed previous reports that resveratrol reduces melanin synthesis by reducing the activity of the rate-limiting enzyme tyrosinase. In contrast, the grape extract could not reduce melanin synthesis, and in fact promoted melanogenesis in the presence of α-MSH. The expression of genes related to melanin synthesis, such as tyrosinase, tyrosinase-related protein-1, tyrosinase-related protein-2, and microphthalmia-associated transcription factor, also supports these phenomena, which means that even in the presence of resveratrol, grape extract will strengthen the function of α-MSH in promoting melanin synthesis. Therefore, these results also provide a point of view for research on cosmetics.
黑色素是细胞产生的一种天然色素，可防止紫外线辐射造成的损伤。先前的研究表明，白藜芦醇可减少黑色素的合成。白藜芦醇作为一种具有多种生物活性的天然多酚，存在于多种饮料和植物性食物中，如葡萄。因此，我们研究了含白藜芦醇的葡萄提取物是否也具有调节黑色素合成的能力。在这项研究中，我们使用小鼠 B16F10 黑色素瘤细胞作为黑色素合成模型，用促黑色素生成的α-促黑素细胞激素（α-MSH）作为阳性对照。我们的结果证实了先前的报道，即白藜芦醇通过降低限速酶酪氨酸酶的活性来减少黑色素的合成。相比之下，葡萄提取物不能减少黑色素的合成，实际上在α-MSH 存在的情况下促进了黑色素生成。与黑色素合成相关的基因的表达，如酪氨酸酶、酪氨酸酶相关蛋白-1、酪氨酸酶相关蛋白-2 和小眼畸形相关转录因子，也支持这些现象，这意味着即使存在白藜芦醇，葡萄提取物也会增强α-MSH 促进黑色素合成的功能。因此，这些结果也为化妆品研究提供了一个观点。
