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选择性靶向鸡CD83的血凝素抗原克服了母源抗体对鸡的干扰。

Haemagglutinin antigen selectively targeted to chicken CD83 overcomes interference from maternally derived antibodies in chickens.

作者信息

Shrestha Angita, Meeuws Rick, Sadeyen Jean-Remy, Chang Pengxiang, Van Hulten Marielle, Iqbal Munir

机构信息

The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, UK.

Department of Zoology, Peter Medawar Building, South Parks Road, University of Oxford, Oxford, OX1 3SY, UK.

出版信息

NPJ Vaccines. 2022 Mar 3;7(1):33. doi: 10.1038/s41541-022-00448-2.

DOI:10.1038/s41541-022-00448-2
PMID:35241682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8894371/
Abstract

Maternally derived antibodies (MDAs) are important for protecting chickens against pathogens in the neonatal stage however, they often interfere with vaccine performance. Here, we investigated the effects of MDAs on a targeted antigen delivery vaccine (TADV), which is developed by conjugating H9 subtype avian influenza virus haemagglutinin (HA) antigen to single chain fragment variable (scFv) antibodies specific for the chicken antigen presenting cell receptor CD83. Groups of 1-day-old chickens carrying high levels of MDAs (MDA++) and 14-day old chickens carrying medium levels of MDAs (MDA+) were immunised with TADV (rH9HA-CD83 scFv), untargeted rH9HA or inactivated H9N2 vaccines. Immunogenicity in these vaccinated chickens was compared using haemagglutination inhibition (HI) and enzyme-linked immunosorbent assays (ELISA). The results showed that the TADV (rH9HA-CD83 scFv) induced significantly higher levels of H9HA-specific antibody titres compared to the untargeted rH9HA and inactivated H9N2 vaccines in MDA++ and MDA+ chickens. Overall, the data demonstrates immune responses induced by TADV are not affected by the MDA in chickens.

摘要

母源抗体（MDA）对于保护雏鸡在新生阶段抵御病原体很重要，然而，它们常常会干扰疫苗的效果。在此，我们研究了MDA对一种靶向抗原递送疫苗（TADV）的影响，该疫苗是通过将H9亚型禽流感病毒血凝素（HA）抗原与针对鸡抗原呈递细胞受体CD83的单链可变片段（scFv）抗体偶联而研发的。将携带高水平MDA的1日龄雏鸡组（MDA++）和携带中等水平MDA的14日龄雏鸡组（MDA+）用TADV（rH9HA-CD83 scFv）、非靶向rH9HA或灭活H9N2疫苗进行免疫。使用血凝抑制（HI）和酶联免疫吸附测定（ELISA）比较这些接种疫苗雏鸡的免疫原性。结果显示，与非靶向rH9HA和灭活H9N2疫苗相比，TADV（rH9HA-CD83 scFv）在MDA++和MDA+雏鸡中诱导产生的H9HA特异性抗体滴度显著更高。总体而言，数据表明TADV诱导的免疫反应不受雏鸡体内MDA的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/5a50251ebaf5/41541_2022_448_Fig8_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/8af2fbe0535c/41541_2022_448_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/9d02daba4666/41541_2022_448_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/5a50251ebaf5/41541_2022_448_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/ca14c80dbd43/41541_2022_448_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/5fbe5845a297/41541_2022_448_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/71efc2687085/41541_2022_448_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/8663fbe096d8/41541_2022_448_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/8af2fbe0535c/41541_2022_448_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/2e29ae13bb73/41541_2022_448_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/9d02daba4666/41541_2022_448_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/387d/8894371/5a50251ebaf5/41541_2022_448_Fig8_HTML.jpg

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