Cardenas-Garcia Stivalis, Ferreri Lucas, Wan Zhimin, Carnaccini Silvia, Geiger Ginger, Obadan Adebimpe O, Hofacre Charles L, Rajao Daniela, Perez Daniel R
Department of Population Health, University of Georgia, Poultry Diagnostic and Research Center, Athens, GA 30602, USA.
Southern Poultry Research Group, Watkinsville, GA 30677, USA.
Vaccines (Basel). 2019 Oct 30;7(4):163. doi: 10.3390/vaccines7040163.
Vaccination of hens against influenza leads to the transfer of protective maternally-derived antibodies (MDA) to hatchlings. However, little is known about the transfer of H7N3 vaccine-induced MDA. Here, we evaluated transfer, duration, and protective effect of MDA in chickens against H7N3 HPAIV. To generate chickens with MDA (MDA (+)), 15-week-old White Leghorn hens were vaccinated and boosted twice with an inactivated H7N3 low pathogenic avian influenza virus vaccine, adjuvanted with Montanide ISA 71 VG. One week after the final boost, eggs were hatched. Eggs from non-vaccinated hens were hatched for chickens without MDA (MDA (-)). Both MDA (+) and MDA (-) hatchlings were monitored weekly for antibody levels. Anti-HA MDA were detected by hemagglutination inhibition assay mostly until day 7 post-hatch. However, anti-nucleoprotein MDA were still detected three weeks post-hatch. Three weeks post-hatch, chickens were challenged with 10 EID/bird of Mexican-origin H7N3 HPAIV. Interestingly, while 0% of the MDA (-) chickens survived the challenge, 95% of the MDA (+) chickens survived. Furthermore, virus shedding was significantly reduced by day 5 post-challenge in the MDA (+) group. In conclusion, MDA confers partial protection against mortality upon challenge with H7N3 HPAIV, as far as three weeks post-hatch, even in the absence of detectable anti-HA antibodies, and reduce virus shedding after challenge.
给母鸡接种流感疫苗可导致保护性母源抗体（MDA）传递给雏鸡。然而，关于H7N3疫苗诱导的MDA的传递情况却知之甚少。在此，我们评估了鸡体内MDA针对H7N3高致病性禽流感病毒（HPAIV）的传递、持续时间及保护效果。为培育具有MDA的鸡（MDA(+)），对15周龄的白来航母鸡接种并用Montanide ISA 71 VG佐剂的灭活H7N3低致病性禽流感病毒疫苗进行两次加强免疫。最后一次加强免疫一周后，孵化鸡蛋。将未接种疫苗的母鸡所产鸡蛋孵化用于培育无MDA的鸡（MDA(-)）。每周监测MDA(+)和MDA(-)雏鸡的抗体水平。通过血凝抑制试验检测抗血凝素（HA）MDA，大多在雏鸡出壳后第7天可检测到。然而，抗核蛋白MDA在出壳后三周仍可检测到。出壳后三周，用每只10个鸡胚感染剂量（EID）的墨西哥源H7N3 HPAIV对鸡进行攻毒。有趣的是，MDA(-)组鸡在攻毒后0%存活，而MDA(+)组鸡95%存活。此外，MDA(+)组在攻毒后第5天病毒排泄显著减少。总之，MDA在雏鸡出壳后长达三周的时间里，即使在未检测到抗HA抗体的情况下，也能对H7N3 HPAIV攻毒提供部分保护作用，减少攻毒后的病毒排泄。
