Ren Zhihua, Jia Guilin, He Hongyi, Ding Ting, Yu Yueru, Zuo ZhiCai, Hu Yanchun, Zhong Zhijun, Yu Shumin, Deng Huidan, Shen Liuhong, Cao Suizhong, Peng Guangneng, Wang Ya, Cai Dongjie, Gou Liping, Ma Xiaoping, Liu Haifeng, Zhou Ziyao, Deng Youtian, Yang Dingyong, Deng Junliang
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
College of Animal Husbandry and Veterinary Medicine, Chengdu Agricultural College, Chengdu, China.
Front Microbiol. 2022 Feb 15;13:846747. doi: 10.3389/fmicb.2022.846747. eCollection 2022.
Porcine deltacoronavirus (PDCoV) is an emerging porcine intestinal coronavirus in recent years, which mainly causes different degrees of vomiting and diarrhea in piglets and has caused great harm to the swine husbandry worldwide since its report. Selenium is an essential trace element for organisms and has been demonstrated to have antiviral effects. In this study, pig kidney epithelial (LLC-PK) cells were used to study the antiviral activity of selenomethionine (Se-Met) (2, 4, 8, and 16 μM) against PDCoV by detecting the replication of the virus, the expression of the mitochondrial antiviral signal protein (MAVS) protein, and the phosphorylation of interferon regulatory factor-3 (IRF-3), IFN-α, and IFN-β, and the changes in glutathione content, glutathione peroxidase, superoxide dismutase activity, and hydrogen peroxide content in the cells. The results showed that Se-Met at higher than physiological concentrations (16 μM) could significantly inhibit the replication of PDCoV in LLC-PK cells and enhance the expression of MAVS protein and the phosphorylation of IRF-3. In addition, Se-Met also improved the intracellular production of IFNα/β and antioxidant capacity with increasing doses. These data suggest that the availability of selenium through selenomethionine supports the antiviral response in porcine kidney cells, and the specific mechanism is attributed to the improved cellular antioxidant capacity and activation of the MAVS pathway by Se-Met.
猪德尔塔冠状病毒（PDCoV）是近年来出现的一种猪肠道冠状病毒，主要导致仔猪不同程度的呕吐和腹泻，自其被报道以来，已对全球养猪业造成了巨大危害。硒是生物体必需的微量元素，已被证明具有抗病毒作用。在本研究中，通过检测病毒复制、线粒体抗病毒信号蛋白（MAVS）蛋白的表达、干扰素调节因子-3（IRF-3）、IFN-α和IFN-β的磷酸化，以及细胞内谷胱甘肽含量、谷胱甘肽过氧化物酶、超氧化物歧化酶活性和过氧化氢含量的变化，利用猪肾上皮（LLC-PK）细胞研究了硒代蛋氨酸（Se-Met）（2、4、8和16μM）对PDCoV的抗病毒活性。结果表明，高于生理浓度（16μM）的Se-Met可显著抑制PDCoV在LLC-PK细胞中的复制，并增强MAVS蛋白的表达和IRF-3的磷酸化。此外，Se-Met还随着剂量增加提高了细胞内IFNα/β的产生和抗氧化能力。这些数据表明，通过硒代蛋氨酸提供的硒支持猪肾细胞中的抗病毒反应，具体机制归因于Se-Met改善了细胞抗氧化能力并激活了MAVS途径。
